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机构地区:[1]宁波市妇女儿童医院,315012
出 处:《中国优生与遗传杂志》2001年第6期58-59,共2页Chinese Journal of Birth Health & Heredity
摘 要:目的 :了解我国妊娠与非妊娠妇女生殖道肺炎支原体 (Mpn)感染状况。方法 :应用套式聚合酶链式反应(nPCR)、选择性培养和全自动DNA序列测定技术对 10 0例妊娠妇女和 10 0例非妊娠妇女的阴道拭子标本进行检测。结果 :MpnnPCR检测妊娠妇女阴性率为 6 % ;非妊娠妇女为 3%。MpnnPCR呈阳性的标本经选择性培养均分离到Mpn。 1例MpnnPCR阳性产物经全自动DNA序列测定与Mpn标准株序列完全一致。结论 :我国妊娠与非妊娠妇女生殖道均存在Mpn感染。Objective:To study mycoplasma pneumoniae(Mpn) infection in the genital path of pregnant women and non-pregnant women in china.Methods Applying nested Polymerase Chain Reaction(nPCR)?selective cultrure and whole automatism DNA sequence mensuration technique determined vagina speciens from 100 pregnant women and 100 non-pregnant women.Results Postive rate of Mpn in pregnant women was 6% with nPCR;Postive rate of Mpn in non-pregnant women was 3% with nPCR.Among the postive cases,Mpn were detected with selective culture.Sequence of 1 Mpn nPCR postive product was entire consistent with MPn standard.Conclution There exsists Mpn infection in the genital path of pregnant women and non-pregnant women in china.
分 类 号:R195.4[医药卫生—卫生统计学]
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