清肝冲剂对小鼠急性肝损伤治疗作用的观察  

Therapeutic Action of Qinggan Granule for Acute Liver Injury

作　　者：张斌[1] 王灵台[1] 陈建杰[1] 张铭[1] 林益群[1] 

机构地区：[1]上海中医药大学附属曙光医院,上海200021

出　　处：《中国中西医结合消化杂志》2001年第6期339-340,344,共3页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

摘　　要：目的 :研究清肝冲剂对小鼠急性肝损伤的治疗作用。方法 :分别采用四氯化碳 (CCl4 )和D 氨基半乳糖腹腔内注射造成小鼠急性肝损伤 ,清肝冲剂治疗组小鼠于造模 2h后用清肝冲剂灌胃给药连续 3d ,然后检测肝功能 ,观察肝脏病理变化。结果 :CCl4 及D 氨基半乳糖造模后 ,造成小鼠急性肝脏损伤 ,出现明显肝功能异常。用清肝冲剂治疗 3d后 ,CCl4 模型治疗组小鼠丙氨酸转氨酶 (ALT)和天冬氨酸转氨酸 (AST)较CCl4 模型组均有所降低 ,但差异无显著性意义 (P >0 .0 5 )。D 氨基半乳糖模型给药组的小鼠AST、AST ALT、白蛋白、球蛋白 (A、G)等肝功能指标与D 氨基半乳糖模型组比较都有明显的改善 (P <0 .0 1)。病理观察清肝冲剂治疗组小鼠肝细胞变性、肿胀程度较模型组减轻。结论 :清肝冲剂对CCl4 和D 氨基半乳糖造成小鼠急性肝损伤模型具有改善肝功能、减轻肝脏病理损害的作用。Objective:To further assess the efficiency of Qinggan granule(QGG) on protection hepatocytes from acute injury in animal model.Methods:Acute liver injury were induced by injection of carbon tetrachloride and D aminogalactose into peritoneal cavity respectively.Two hours later,QGG were given orally constant for three days.Then mice were killed,liver function test and pathological changes were observed.Results:Liver function tests were abnormal rapidly and significantly after injection of carbon tetrachloride and D aminogalactose in control group,after treatment of QGG,the level of serum ALT and AST decreased significantly in D aminogalactose group(P< 0.01 ),but a slight decrease in carbon tetrachloride group(P> 0.05 ).Pathologically,the degeneration and swollen of hepatocytes were attenuated obviously in QGG group.Conclusion:QGG could prevent hepatocytes from injury induced by carbon tetrachloride and D aminogalactose,it can improve recovery of liver function and attenuate tissue damage in liver.

关 键 词：丙型肝炎 肝损伤 清肝冲剂 中医药疗法 

分 类 号：R259.126.3[医药卫生—中西医结合]