尾部悬吊大鼠胸主动脉及肺动脉内皮细胞一氧化氮合酶mRNA表达的动态变化  

The Dynamic Changes of NOSmRNA in Endothelial Cells of Aortae and Pulmonary Arteries in Rats under Tail Suspension.

作　　者：汪德生[1] 孙磊[1] 任维[1] 董建文[1] 马铁民[1] 向求鲁[1] 

机构地区：[1]航天医学工程研究所,北京100094

出　　处：《航天医学与医学工程》2001年第5期318-322,共5页Space Medicine & Medical Engineering

摘　　要：目的观察模拟失重时大小循环动脉血管内皮细胞一氧化氮合酶mRNA(NOSmRNA)表达随时间变化的过程 ,为模拟失重时血管局部调节的适应机理研究提供资料。方法采用Wistar大鼠 30°尾部悬吊模拟失重效应 ,原位杂交技术观察悬吊 7d(TS7组 )、1 4d(TS1 4组 )及对照 (CON组 )胸主动脉、肺动脉内皮细胞eNOSmRNA和iNOSmRNA表达变化。结果TS7组胸主动脉和肺动脉内皮细胞eNOSmRNA和iN OSmRNA的升高非常显著 ;TS1 4组肺动脉内皮细胞eNOSmRNA的升高仍非常显著而胸主动脉回到对照水平 ,TS1 4组胸主动脉内皮细胞iNOSmRNA回到对照水平而肺动脉下降非常显著。结论模拟失重对大循环动脉内皮细胞eNOSmRNA和iNOSmRNA的影响趋势相似 ,而对肺循环动脉影响趋势不同 ,归因于模拟失重初期由下体转移而来的体液进入大小循环高峰期的时间过程不同 ,可能是模拟失重时局部调节功能降低的一种重要表现 。Objective Through the observations of dynamic changes of eNOSmRNA and iNOSmRNA in arterial endothelial cells of systematic circulation and pulmonary circulation under simulated weightlessness, to collect some data for studies of the adaptive mechanisms of local regulation in arterial systems. Method Wistar rats were -30° tail suspended to simulate the effects of weightlessness. The rats were randomly divided into three groups: control group(CON),7 day tail suspension group(TS7) and 14 day tail suspension group(TS14). Changes of NOSmRNA expresses in endothelial cells of the thoracic aortae and pulmonary arteries were observed with in situ hybridization technique. Result The eNOSmRNA and iNOSmRNA of thoracic aortic and pulmonary arterial endothelial cells in TS7 rats increased very significantly. The eNOSmRNA of thoracic aortic endothelial cells from TS14 rats returned to control level, but remained very significantly increased in pulmonary arteries. The iNOSmRNA in pulmonary arterial endothelial cells from TS14 rats decreased very significantly, but that in thoracic aortae returned to the control level. Conclusion The responses of eNOSmRNA and iNOSmRNA in arterial endothelial cells of systematic circulation to tail suspension were similar, but they were different in pulmonary arterial endothelial cells, which might be due to the difference in the peak course of the shift of fluid from lower body entering the pulmonary or systematic circulation during initial period of simulated weightlessness. It could be a kind of important sign of depressed local regulative function under simulated weightlessness and might contribute to orthostatic intolerance after simulated weightlessness.

关 键 词：尾悬吊 主动脉 胸 内皮细胞 肺动脉 一氧化氮合酶 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]