自身免疫性血小板减少性紫癜的特异性免疫学诊断的研究  

The diagnostic value of platelet glycoprotein-specific autoantibody detection in autoimmune thrombocytopenic purpura

作　　者：李蔚[1] 侯明[1] 张茂宏[1] 朱媛媛 马道新[2] 

机构地区：[1]山东大学齐鲁医院(原山东医科大学附属医院)血液内科,济南250012 [2]山东大学齐鲁医院血液病研究室

出　　处：《中华血液学杂志》2001年第7期374-376,共3页Chinese Journal of Hematology

基　　金：山东省科委基金资助项目 ( 970 0 10 );山东省优秀青年科学家基金资助项目 ( 973 7442 8)

摘　　要：目的　检测自身免疫性血小板减少性紫癜 (AITP)患者及非免疫性血小板减少症患者的抗血小板特异性抗体 ,并与血小板相关抗体 (PAIgG)相比较 ,评价其诊断及鉴别诊断价值。方法　用酶联免疫吸附竞争法检测PAIgG ,改良单克隆抗体特异性俘获血小板抗原 (MAIPA)技术检测抗血小板GPⅡb/Ⅲa、GPⅠb/Ⅸ的特异性抗体。结果　血小板特异性抗体较PAIgG的敏感性低 ,但特异性明显增强。Objective To evaluate the clinical significance of platelet glycoprotein specific autoantibody detection and platelet associated IgG(PAIgG) measurement in the differential diagnosis between autoimmune thrombocytopenia and nonimmune thrombocytopenias. Methods Anti GPⅡb/Ⅲa and anti GPⅠb/Ⅸ autoantibodies were detected by a modified monoclonal antibody immobilization of platelet antigens assay(modified MAIPA), and PAIgG by competitive ELISA. Results Comparing with the measurement of PAIgG, the GP specific autoantibody assay was less sensitive but much more specific for the diagnosis of autoimmune thrombocytopenia. Conclusion The platelet GP specific autoantibody detection is considerably better than PAIgG measurement in discriminating immune from nonimmune thrombocytopenia.

关 键 词：血小板减少性紫癜 抗原 人血小板 诊断 

分 类 号：R554.6[医药卫生—血液循环系统疾病]