地尔硫对猪心梗面积、降钙素基因相关肽及内皮素的影响  

Effects of diltiazem on myocardial infarct size, calcitonin gene related peptide and endothlin level in pig

作　　者：李醒三[1] 易忠[2] 曾志羽[1] 

机构地区：[1]广西医科大学第一附属医院心内科 [2]广西壮族自治区人民医院心内科,南宁530021

出　　处：《基础医学与临床》2001年第6期554-556,共3页Basic and Clinical Medicine

基　　金：广西壮族自治区卫生厅基金 (0 86 0 2 5 )

摘　　要：本文研究地尔硫 艹卓(Dil)对猪急性心肌梗死 (AMI)面积、降钙素基因相关肽 (CGRP)及内皮素 (ET)的影响。将猪左冠状动脉前降支套扎 6 0min ,恢复血流 180min ,造成急性心肌缺血 /再灌注 (MI/RI)动物模型。随机化分为Dil组 [Dil组 ,Diltiazem 10 0 μg/ (kg·min) ],及对照组 (Con组 ,生理盐水 ) ,均采用冠状静脉逆行灌注法 (CVRI) ,于缺血后5 0min给Dil或生理盐水以 2mL/min速度持续 40min。实验过程中监测心率及平均动脉压变化。测定心肌梗死面积和冠状静脉血浆CGRP及ET含量。结果表明 ,与对照组比较 ,Dil能显著减慢心率 (73 6± 19 8比 110 3± 16 5 ,P <0 0 5 )、减少ET升高浓度 (392 7± 5 0 6比 437 1± 43 1,P <0 0 5 )、降低CGRT下降浓度 (38 2 5± 7 70比 2 9 2 1± 7 76 ,P <0 0 5 )及心肌梗死面积 [(4 0 3± 7 7) %比 (5 9 2± 8 1) % ,P <0 0 5 ]。To explore the effect of calcium antagonist diltiazem on the myocardial infarct size and the plasma levels of calcitonin gene related peptide (CGRP) as well as endothelin (ET) in pigs with acute myocardial infarction. Pig hearts were induced by ligation of the left decending coronary artey for 60 min followed 180 min reperfusion and divided into two groups randomly. Both of them reccived coronary venous retroinfusion and one group received diltiazem [100μg/(kg·min)] as the treatment group. The another one retroinfused saline as control. Diltiazem or saline (2mL/min) was administered for 40 min after 50 min ischemia. During the experiment, the mean artery pressure (MAP) and heant rate (HR) were recorded. The plasma concentration of CGRP and ET in coronary venous and the myocardial infarct sizes were measured. The results showed that, as compared with control group, diltiazem significontly decreased HR ( 73.6±19.8 vs 110.3±16.5, P <0.05), ET levels (292.7±50.6 vs 437.1±43.1, P <0.05), contain higher CGRP (38.25±7.70 vs 29.21±7.76, P <0.05), and reduce myocardial infarct size [(40.3±7.7)% vs (59.2±8.1)%, P <0.05].

关 键 词：地尔硫ZHUO 心梗面积 降钙素基因相关肽 内皮素 猪 

分 类 号：R965[医药卫生—药理学]