益气解毒颗粒对二亚硝基哌嗪诱导人胚鼻咽上皮细胞转化的阻抑作用  

The Inhibitory Effect of YIQIJIEDU Granule on the Transformation of Embryo Nasopharyngeal Epithelia Induced by DNP

作　　者：唐发清[1] 梁日初[1] 田道法[2] 陈朝晖[1] 吴尚辉[3] 段朝军[3] 谌兵来[1] 荆照政[1] 

机构地区：[1]中南大学湘雅医院,湖南长沙410078 [2]湖南中医学院第一附属医院,湖南长沙410007 [3]中南大学湘雅医院肿瘤研究所,湖南长沙410078

出　　处：《湖南中医学院学报》2001年第3期18-20,共3页Journal of Hunan College of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目 ( 39770 933);国家中医药管理局资助项目 ( 95B0 6 3)

摘　　要：目的 :探讨中药复方益气解毒颗粒对人胚鼻咽上皮细胞转化的阻抑作用。方法 :二亚硝基哌 (DNP)诱导人胚鼻咽上皮细胞转化 ,同时用益气解毒颗粒作用于DNP诱导的鼻咽上皮细胞 ,共同培养 ,然后收集不同代数细胞 ,酶连免疫聚合酶链反应 (PCR -ELISA)和巢式聚合酶链反应 (Nested -RT -PCR)法检测实验细胞端粒酶活性和端粒酶RNA的表达。结果 :加益气解毒颗粒药液组鼻咽细胞形态明显好于盐水对照组 ,其端粒酶活性 (0 34± 0 19)也明显低于对照组 (0 73± 0 2 7) (P <0 0 5 )。结论 :益气解毒颗粒能够抑制鼻咽上皮细胞端粒酶的激活 ,进而阻断DNP诱导的细胞转化。To investigate the inhibitory effect of YIQIJIEDU Granule on the transformation of embryo nasopharyngeal epithelia induced by N,N'Dinitrosopiperazine(DNP) Methods:Nasopharyngeal epithelia from human embryo induced with water beg at 4～7 months were cultured together with DNP and YIQIJIEDU Granule for 24 h Then,the cells were collected for the observation of morphology and assays of telomerase activity and telomerase RNA expression with the techniques of PCR-ELISA and Nested-RT-RCP respectively Results:The cells incubated wity YIQIJIEDU Granule had much better morphology than those in the control group,and significantly decreased telomerase activity(0 34±0 19) compared with the control group (0 37± 0 27) Conclusion: YIQIJIEDU Granule can greatly prevent the normal nasopharyngeal epithelia from transforming induced by DNP through the inhibition of telomerase activation

关 键 词：鼻咽细胞 端粒酶 RNA 癌变 益气解毒颗粒 黄连 

分 类 号：R285.5[医药卫生—中药学] R289.5[医药卫生—中医学]