新风胶囊对佐剂性关节炎大鼠滑膜细胞及脾脏淋巴细胞超微结构作用的实验研究  

Experimental Study on Effect of Xinfeng Capsule in Treating Rats′ Adjuvant Arthritis on Ultrastructure of Synoviocyte and Splenic Lymphocyte

作　　者：刘健[1] 郑志坚[2] 方朝晖[1] 崔宜武[1] 雷黎[1] 胡闻[3] 韩明向[1] 

机构地区：[1]安徽中医学院第一附属医院,合肥230031 [2]卫生部北京医院 [3]安徽省立医院

出　　处：《中国中西医结合杂志》2001年第12期917-919,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：卫生部优秀青年科研基金资助项目 (No.992 6)

摘　　要：目的 :观察新风胶囊对佐剂性关节炎大鼠治疗作用及对滑膜细胞、脾脏淋巴细胞超微结构的作用。方法 :将 6 0只大鼠随机分为正常对照组、模型对照组、新风胶囊组和雷公藤组 ,每组 15只 ,制备完全佐剂并分别向除正常对照组以外其余动物右后足跖皮内注射 0 1ml致炎。电镜观察滑膜细胞、脾脏淋巴细胞超微结构变化 (包括线粒体肿胀、空泡变、嵴突病变等 ) ,计算各组线粒体病变率。结果 :与治疗前比较 ,新风胶囊组及雷公藤组治疗后右后足跖肿胀度显著降低 (P <0 0 5 ) ;与模型对照组比较 ,新风胶囊组和雷公藤组治疗后模型大鼠关节滑膜细胞线粒体的病变率显著降低 (P <0 0 5 ) ;新风胶囊组脾脏淋巴细胞线粒体病变率显著降低 (P <0 0 5 ) ,而雷公藤组脾脏淋巴细胞线粒病变率差异无显著性 (P >0 0 5 )。与雷公藤组比较 ,新风胶囊组治疗后滑膜细胞及脾脏淋巴细胞线粒体病变率显著降低 (P <0 0 5 )。结论 :新风胶囊与雷公藤均能降低佐剂性关节炎大鼠病变关节的肿胀度 ,新风胶囊改善佐剂性关节炎大鼠滑膜和脾脏的超微结构病变的作用优于雷公藤 。Objective: To observe the effect of Xinfeng capsule (XFC) on the ultrastructure of synoviocyte and splenic lymphocyte in rat adjuvant arthritis (AA) model. Methods: Sixty rats were randomly divided into normal control group, model group, XFC group and Tripterygium wilfordii polycoside (TP) group, 15 in each. Complete adjuvant 0.1 ml was injected into right posterior metatarsus of rats subcutaneously to induce inflammation, except that in the normal control. The ultrastructural change including mitochondria swelling, vacuolation and ridge pathologic change of synoviocyte and splenic lymphocyte was observed by transmission electron microscopy (TEM). The mitochondria lesion rate (MLR) of each group was then calculated. Results: The swelling degree of right posterior metatarsus in XFC and TP group obviously lowered after treatment (P<0 05). MLR of synoviocyte in the XFC group and the TP group after treatment was also obviously lower than that in the model group (P<0 05). MLR of splenic lymphocyte in the XFC group reduced markedly (P<0 05) after treatment, while in the TP group, the change was insignificant (P>0 05). In comparison with TP group, the reduction of both MLR of synoviocyte and splenic lymphocyte after treatment in the XFC group was more significant (P<0 05). Conclusion: XFC could lower the degree of swelling in AA rats like TP did, but XFC could improve ultrastructural change of rats′ synoviocyte and splenic lymphocyte better than that of TP, which was likely the morphological basis of XFC′s therapeutical effect.

关 键 词：新风胶囊 佐剂性关节炎 滑膜 脾脏 超微结构 中医药 

分 类 号：R259.9[医药卫生—中西医结合]