苦丁茶对豚鼠离体气管平滑肌收缩功能的影响  

The Effect of Kudingcha (Ilex latifolia Thunb.) on the Contraction of Isolated Guinea-pig Tracheal Smooth Muscle in Vitro

作　　者：蒋建敏[1] 许实波[1] 江润祥[2] 

机构地区：[1]中山大学生命科学学院药学系,广东广州510275 [2]香港中文大学中医学院

出　　处：《中国中药杂志》2001年第12期853-856,共4页China Journal of Chinese Materia Medica

基　　金：香港工业署工业发展科技基金项目 (AF/32 3/97)

摘　　要：目的 :研究苦丁茶对豚鼠离体气管平滑肌的影响。方法 :以累积技术建立CaCl2 和组织胺 (His)的量效曲线 ,研究了苦丁茶对CaCl2 ,His和乙酰胆碱 (Ach)等致喘介质对豚鼠离体气管平滑肌收缩功能的影响。结果 :CaCl2 和His引起气管条明显的收缩 ,pD2 分别为 3.5 9和 5 .34。苦丁茶能明显压低CaCl2 和His的量效曲线 ,并使曲线右移。苦丁茶对Ach和His 3× 10 -6mmol·L-1引起的气管平滑肌收缩有明显的逆转作用 ,IC50 分别为 0 .16mg·ml-1和 0 .2 1mg·ml-1。结论Objective: To study the effects of Kudingcha ( Ilex latifolia Thunb., IL) on the isolated guinea pig tracheal smooth muscle in vitro. Method: By obtaining CaCl 2 and Histamine accumulative dose response curves, to observe the influences of IL on the contraction of tracheal strips induced by calcium and some asthmogenic mediators. Result: CaCl 2 and histamine caused significant contraction of tracheal smooth muscle and pD 2 was 3.55 and 5.34 respectively. After incubated with IL, the dose response curves of CaCl 2 and histamine were significantly shifted to the right, and the maximal contractile force was reduced. IL could also inhibit isolated tracheal strip contraction induced by acetylcholine 3×10 -6 mol·L -1 and histamine 3×10 -6 mol·L -1 , and IC 50 was 0.16mg·ml -1 and 0.21mg·ml -1 . Conclusion: Kudingcha has significant dilated effects on tracheal smooth muscle.

关 键 词：苦丁茶 氯化钙 组织胺 乙酰胆碱 

分 类 号：R285.5[医药卫生—中药学] R282.710.5[医药卫生—中医学]