新试剂咪唑偶氮对甲基苯与钴的显色反应研究  

Study of color reaction of cobalt with a new chromogenic reagent IAP-4M by atomic absorption spectrometry

作　　者：邵红[1] 夏心泉[1] 范文玉[1] 

机构地区：[1]沈阳化工学院,沈阳110021

出　　处：《分析试验室》2001年第5期27-29,共3页Chinese Journal of Analysis Laboratory

摘　　要：合成了一种新的杂环偶氮类显色剂 -咪唑偶氮对甲基苯 ( IAP- 4M)。用原子吸收分光光度计代替紫外 -可见分光光度计 ,以铁空心阴极灯为光源 ,系统研究了该试剂与钴的显色反应条件。应用于维生素 B12 中钴含量的测定 ,RSD=1 .34% ,回收率为 98%A new chromogenic reagent 2 ( 2' imidazolylazo) 4 methyl phenyl(IAP 4M) was synthesized and used for determination of cobalt by spectrophotometry. The results showed that in the presence of Triton x 100 and in NH 3·H 2O NH 4Cl buffer (pH 9.8～10.8), the red Co(Ⅱ) IAP 4M complex is formed .The apparent molar absorptivity ε is 1.91×10 5 L·moL -1 ·cm -1 . Beer's law is obeyed in the range of 0～2.5 μg/25 mL for Co(Ⅱ). The 440.0 nm spectral line emitted by the iron lamp and the atomic absorption spectrophotometer were employed. The method was applied to determine micro amounts of cobalt in Vitamin B 12 .

关 键 词：原子吸收分光光度计 紫外-可见分光光度计 显色反应 分光光度法 维生素B12 测定 钴 2-(2'-咪唑偶氮)-4-甲基苯 

分 类 号：O614.812[理学—无机化学] Q563[理学—化学]