用低交换容量聚苯乙烯型强酸性阳离子交换树脂柱色谱分离中性氨基酸  

Separation of Neutral Amino Acids on Columns Packed with Polystyrene-Based Strongly Acidic Cation Exchange Resins with Low Ion Exchange Capacity

作　　者：刘菊湘[1] 刘国栋[1] 阎虎生[1] 程晓辉[1] 何炳林[1] 姜根华[2] 杨国英[2] 

机构地区：[1]南开大学高分子化学研究所吸附与分离功能高分子材料国家重点实验室,天津300071 [2]天津药业有限公司,天津300161

出　　处：《高等学校化学学报》2001年第12期2100-2103,共4页Chemical Journal of Chinese Universities

基　　金：天津市重点科学基金项目 (批准号 :98380 14 11)资助

摘　　要：通过磺化苯乙烯 -二乙烯苯共聚物或商品化的聚苯乙烯型强酸性阳离子交换树脂 ( 0 0 1× 7)的逆磺化反应 ,得到一系列不同交换容量的聚苯乙烯型强酸性阳离子交换树脂 .研究了丙氨酸和缬氨酸及缬氨酸和亮氨酸在这些树脂柱上的色谱分离 .结果表明 ,用两种方法得到的树脂对丙氨酸和缬氨酸的色谱分离性能基本相同 ;同时中性氨基酸与聚苯乙烯型强酸性阳离子交换树脂之间的作用包括离子作用和疏水作用 ,且二者之间存在协同作用 .A series of polystyrene\|based strongly acidic cation exchange resins with different ion exchange capacities were synthesized \%via\% the reversed\|sulfonation of a commercial polystyrene\|based strongly acidic cation exchange resin(001×7) or sulfonation of styrene\|divinylbenzene copolymer. The chromatographic separation of alanine and valine or valine and leucine on columns packed with these resins was carried out. The results showed that the chromatographic properties of the two kinds of the above\|mentioned resins on the separation of alanine and valine were almost the same. The retention volume of alanine was less than that of valine on the same column with various ion exchange capacities, and the retention volume of valine was less than that of leucine on the column with the ion exchange capacity of 1.67 mmol/mL. The retention volume of alanine decreased when the ion exchange capacities decreased from \{2.02\} to \{1.00 mmol/mL\}. The retention volume of valine slightly decreased when the ion exchange capacities decreased from \{2.02\} to 1.67 mmol/mL and remained almost unchanged when the ion exchange capacities decreased from \{1.67\} to 1.00 mmol/mL. These results indicated that the interaction between neutral amino acids and the polystyrene\|based strongly acidic cation exchange resin includes ionic and hydrophobic interactions. And there may be synergy between the two interactions. The better separation efficiency of neutral amino acid may be obtained by using the resin with a lower ion exchange capacity.

关 键 词：氨基酸 阳离子交换树脂 疏水作用 色谱 聚苯乙烯 分离 性能 色谱柱 蛋白质水解液 

分 类 号：TQ287.11[化学工程—有机化工] TQ028.33