大孔吸附树脂对肿瘤坏死因子吸附性能的研究  

Studies on Adsorption of TNF α with Macroporous Resin

作　　者：魏佼[1] 俞耀庭[1] 

机构地区：[1]南开大学生物活性材料教育部重点实验室,天津300071

出　　处：《高等学校化学学报》2001年第11期1843-1845,共3页Chemical Journal of Chinese Universities

基　　金：中奥合作项目 (天津市国际合作 )基金;国家自然科学基金 (批准号 :30 0 0 0 0 42 )资助

摘　　要：选用 NK-1 1 0、碳化树脂和 MET-1 0 0 4 3种大孔吸附树脂 ,通过对树脂吸附量的测定 ,吸附动力学曲线和吸附等温线的描述等方法 ,研究了 3种大孔吸附树脂对血浆中 TNFα的吸附性能 ,结果表明 NK-1 1 0和 MET-1 0 0 4对 TNFα的吸附量较高 ,其中又以 MET-1 0 0There are increasing evidences that high serum levels of Tumor Necrosis Factor(TNF α) have been shown to be associated with disease severity in fulminant hepatic failure and dyscrasia, which is a principal mediator in the cascade of pathophysioligic events following endotoxic shock. In the present study, NK 110, carbonized resin and MET 1004 are selected as adsorbents in the static adsorption experiments of TNF α. We have investigated the adsorption capacity, kinetic profiles and adsorption isotherm of the three macroporous resins. Experimental results show that MET 1004 and NK 110 have higher adsorption capacities than carbonized resin. Compared with NK 110, the velocity of adsorption of MET 1004 towards TNF α is much faster than the former. From experimental data the adsorption rate constants k are calculated to be 0.016 3 min -1 (NK 110); 0.018 9 min -1 (carbonized resin); 0.007 9 min -1 (MET 1004) at 37 ℃, respectively. Furthermore, their adsorption isotherms are also in different shapes. They are shown to be 'H'(MET 1004); 'L'(NK 110); 'S'(carbonized resin) at 37 ℃, respectively. This demonstrated that the surface to volume ratio and the pore size of macroporous resins had an strong impact on the adsorption capacity and as the pore size increased, the adsorptive rate increased.

关 键 词：大孔吸附树脂 肿瘤坏死因子 吸附 血浆 内毒素休克 治疗 多脏器功能衰竭 全血灌流 吸附剂 

分 类 号：R631.4[医药卫生—外科学] R457[医药卫生—临床医学]