HLA-Ⅰ、Ⅱ类PCR-SSP基因分型在移植配型及亲子鉴定中的应用  

Application of PCR-SSP in transplantation typing and Consanguinity Identify

作　　者：周永安[1] 乔巨[1] 朱镭[1] 张丽[1] 鹿育晋[1] 

机构地区：[1]山西医科大学第二医院,山西太原030001

出　　处：《山西临床医药》2001年第12期885-887,共3页Shanxi Clinical Medicine

摘　　要：目的：建立快速、准确的ＨＬＡ基因分型方法，满足临床移植配型的需要。方法：对２５对已进行ＨＬＡ抗原血清学分型的骨髓移植的供、受者，１２例亲子鉴定应用聚合酶链反应－序列特异引物（ＰＣＲ－ＳＳＰ）进行ＨＬＡ－Ⅰ、Ⅱ类基因Ａ、Ｂ、ＤＲＢ１、ＤＱＢ１位点扩增，琼脂糖凝胶电泳分析ＰＣＲ产物并确定其基因型。结果：基因分型结果与血清学分型一致者有２２对，基因分型能明显判断而血清学分型无法判断者有３对；排除亲子关系的有２例。结论：ＰＣＲ－ＳＳＰ方法具有操作简单、快速、结果可靠的特点，不仅适用于移植配型、法医学亲子鉴定和个人识别，也可用于相关疾病及人类遗传学的研究。Objective: To establish a rapid,accurate HLA DNA typing method in application of NMT; Methods: HLA genotyping was performed by using PCR - SSP in 25 pairs of bone marrow transplantation donor - recipients who underwent serologic typing and 12 Consanguinity Identify,and identified their genotype by analysis of amplification products agarose gel electrophoresis. Results :The serological and genotyping results were identical in 22 cases, serologic typing was failure in 3 cases who could be typed easily by genotyping methods. It can identify 2 cases non-Consanguinity. Conclusion:The method is simple,fast .accurate. Its result is available. It can not only apply to transplant typing,Consanguinity Identifying in medical jurisprudence and individual identifying but also research associated disease and human heredity genetics.

关 键 词：HLA PCR-SSP 移植配型 亲子鉴定 基因分型 

分 类 号：Q343[生物学—遗传学]