日本血吸虫中国大陆株62kDa重组抗原的表达、纯化及鉴定  

EXPRESSION,PURIFICATION AND IDENTIFICATION OF RECOMBINANT 62 kDa PROTEIN OF SCHISTOSOMA JAPONICUM

作　　者：马磊[1] 苏川[1] 胡雪梅[1] 季旻珺[1] 吴海玮[1] 陈淑贞[1] 张耀璧[2] Martin G Taylor 张兆松[1] 

机构地区：[1]南京医科大学寄生虫学教研室,南京210029 [2]伦敦大学热带病与公共卫生学院

出　　处：《中国血吸虫病防治杂志》2001年第5期265-267,共3页Chinese Journal of Schistosomiasis Control

基　　金：总理预备金血吸虫病疫苗研究项目专项资助 ( NO:94-Y-2 3)

摘　　要：目的　获得并鉴定日本血吸虫 6 2 k Da重组抗原。方法　将 Sj6 2 / p GEX- 1λT/ TG2 克隆菌用 IPTG诱导表达 ,经超声粉碎 ,离心后取上清过 GS4B柱 ,用还原型谷胱甘肽洗脱液洗脱后获得纯化的 r Sj6 2 / 2 6 GST重组融合蛋白 ,用 Throm bin酶切后获得纯化的 r Sj6 2重组蛋白 ,并对重组蛋白进行 SDS- PAGE和 Western blotting鉴定。结果　重组蛋白纯度较高 ,且可被血吸虫感染的鼠血清识别。Objective To obtain and identify a purified 62 kDa recombinant protein (rSj 62). Methods The Sj 62/pGEX-1λT/TG 2 positive recombinant clone was expressed by inducing with IPTG and ultrasonicated. The rSj 62/26 GST fusion protein could be purified with GS4B beads, from which the rSj62 protein could be obtained by digestion with Thrombin. The recombinant proteins were identified by SDS-PAGE and Western blotting. Results The recombinant proteins had good purity and could be recognized by the sera from the mice infected with Schistosoma japonicum. Conclusion The purified rSj 62 and rSj 62/26 GST had the same immunological activity as the native worm protein.

关 键 词：日本血吸虫 rSj26 中国大陆株 重组抗原 

分 类 号：R383.24[医药卫生—医学寄生虫学]