先天性心脏病心肌组织中HPVB19,RV基因的检测  

Detection of HPVB19 and RV gene in cardiac tissue from children with congenital heart disease

作　　者：张国成[1] 王晓明[1] 许东亮[1] 孙新[1] 李飚[1] 

机构地区：[1]第四军医大学西京医院儿科

出　　处：《第四军医大学学报》2001年第21期1957-1960,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目 ( 39870 0 2 1)

摘　　要：目的　探讨 HPVB19和 RV等病原感染与先心病的发病关系 .方法　采用 PCR,RT- PCR及 ISH等分子生物技术 ,对 79例先心病和 46例非先心病的先心病心肌组织中HSVB19,RV,TOX等病原基因进行检测及 B19病毒在心肌细胞内定位研究 .结果　 1在先心病心肌组织中 B19,TOX,RV,HSV病原基因的检测阳性率分别为 18% (12 / 6 6 ) ,13%(10 / 79) ,2 3% (7/ 30 )和 4% (3/ 79) ,前三者与对照组间比较有显著性差异 (P<0 .0 5 ) ,而 HSV在两组间无统计学意义 (P>0 .0 5 ) ,提示 B19,TOX,RV为先心病发病的重要危险因子 .2对活检心肌组检测上述病原基因的阳性病例 ,同时作血清中 B19,TOX,RV的特异性 Ig M检测均阴性 . 3通过原位杂交显示 B19病毒 DNA主要分布在心肌细胞核内 ,光学显微镜下心肌细胞无炎性浸润 .结论　先心病心肌组织中可检测到 HPVB19,TOX,RV基因 ,并有较高的阳性率 。AIM To explore relationship of HPVB19, Rubella virus (RV) and other pathogeny infection with CHD. METHODS HPVB19, toxoplasma (TOX), RV, Herpes simplex virus (HSV) and other pathogenic gene were detect ed by PCR, RT PCR and B19 viral location was studied in cardiac tissues by ISH in 79 cases of CHD and 46 cases without CHD of children. RESULTS ①The positive rate of B19, Tox, RV and HSV gene in cardiac tissue of CHD was 18% (12/66), 13% (10/79), 23% (7/30) and 4% (3/79) respectively. The positive rate of B19, TOX and RV was significantly different ( P <0.05) between CHD and control group, while no statistical different ( P >0.05) for HSV. The results show that B19, TOX and RV infection might be important risk factors for CHD. ②Specific IgM of B19, TOX and RV is negative from serums of the patients in biopsy group, whose gene detection is positive at the same time. ③B19 DNA is located the nucleus of cardiac cell, which has no inflammation under optical microscope. CONCLUSION The detected rate of HPVB19, TOX and RV in cardiac tissues of CHD patients are significantly different between the two groups, inferring that HPVB19, RV might be correlated with CHD

关 键 词：先天性心脏病 心脏组织 人细小病毒B19 风疹病毒 基因 

分 类 号：R541.1[医药卫生—心血管疾病]