麻疹病毒CC-47株非编码区核苷酸序列测定与比较  

Nucleotide sequence of the noncoding regions of measles virus stain CC-47 and comparison with other measles viruses

作　　者：胡孔新[1] 林崇哲[2] 王晓芳[1] 李川[1] 孟祥芝[1] 李德新[1] 

机构地区：[1]中国预防医学科学院病毒学研究所出血热病毒研究室,北京100052 [2]长春生物制品研究所

出　　处：《中华实验和临床病毒学杂志》2001年第4期327-331,共5页Chinese Journal of Experimental and Clinical Virology

基　　金：国家自然科学基金资助 (3 9970 0 3 6)

摘　　要：目的　测定麻疹病毒疫苗株长 47(CC 47)的非编码区核苷酸序列 ,并和其他麻疹病毒比较 ,为利用疫苗株病毒进一步研究奠定基础。方法　用RT PCR分段扩增并克隆麻疹病毒CC 47株基因组全序列 ,用基因特异引物测定非编码区的核苷酸序列。结果　CC 47疫苗株非编码区在基因组上为 7个不连续的片段 ,总计 1791个核苷酸。与其他野毒株及Edmonston衍生 5株疫苗株非编码区序列比较发现 ,CC 47疫苗株具有与Edmonston疫苗株系列相同的 4个特征性的核苷酸替代位点 ,分别位于基因组 3′末端第 2 6位 (U→A)、42位 (U→G)和M F基因间隔区FmRNA 5′非翻译区的 4978位(A→G)、5 34 9位 (A→G) ,这些位点的改变可能会影响病毒mRNA的合成、加工和翻译效率以及基因组的复制和包装。结论　不同基因型麻疹病毒具有同样的位点改变可能与病毒在半许可细胞中适应或减毒有关 。Objective To determine the nucleotide sequence of noncoding regions of Measles virus strain Changchun 47 (CC 47) and to compare them with other measles virus for revealing some vaccine related information. Methods Six overlapped fragments, that covers complete genome of CC 47 were amplified by using RT PCR, all of the amplified fragments have been cloned and sequenced.Results Seven noncoding regions lie in the genome sequence of CC 47 separated by six structure genes. The noncoding regions of CC 47 contain 1 791 nucleotides totally. Comparing of the noncoding regions of CC 47 with other wild type strains and five Edmonston derived vaccine strains, four nucleotide substitutions were shared by nearly all vaccine strains. Two of these were in the genomic 3′ terminal transcriptional control region:position 26(U→A),42(U→G); the other were in the F mRNA 5′ untranslated region of M/F intergenic region. These site substitutions may influence the efficiency of mRNA synthesis, processing, and translation, as well as genome replication and encapsidation.Conclusion The nucleotide substitutions shared by different genotype measles virus vaccine strains may be related to virus growth in semipermissive cell or process of attenuation, which provide useful data for molecular epidemiology of measles virus. The nucleotide substitutions for attenuation were involved in several regions other than one definite region.

关 键 词：麻疹病毒 非编码区 核苷酸序列 

分 类 号：R373[医药卫生—病原生物学]