肝靶向DNA载体乳糖化多聚乙烯基亚胺及体外转染试验  

Hepatocyte targeted DNA carrier—the lactosylated polyethyleneimine and transfection in vitro?

作　　者：靳雪源[1] 张玲霞[1] 楼敏[1] 谢建芳[1] 

机构地区：[1]解放军第302医院感染六科,北京100039

出　　处：《中华实验和临床病毒学杂志》2001年第4期345-348,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　研制肝靶向的DNA载体 ,用于干扰素的肝细胞内表达或抗病毒反义核酸的肝靶向给药。方法　以还原胺化法制备了肝靶向基因载体乳糖化多聚乙烯基亚胺 (L PEI) ,并进行了体外转染试验和稳定性试验。结果　靶向载体L PEI与DNA形成的复合物 ,对受体阳性的Huh 7细胞具有较高的转染效率 ;靶向载体乳糖化白蛋白能够竞争拮抗这种转染。靶向载体 DNA复合物在大鼠血清中具有较好的稳定性。结论　乳糖化多聚乙烯基亚胺能将DNA选择性的投放于肝细胞 。Objective To develop a hepatocyte targeted DNA carrier for expression of IFN or delivery of antiviral antisense neucleic acid into hapatocytes.Methods The hepatocyte targeted lactosylated polyethleneimine (L\|PEI) was prepared through reductive amination of PEI with lactose. The \%in vitro\% transfection experiment was conducted and the stability of the DNA complex in rat serum was evaluated. Results L\|PEI can effectively deliver luciferase gene into the galactose receptor positive Huh\|7 cells. The transfection can be specifically inhibited by the synthesized ligand\|lactosaminated BSA. The complex of DNA/L\|PEI is stable in rat serum with only little degradation observed after 30 minutes, while free DNA was destroyed completely.Conclusion L\|PEI can be used as an specific and efficient DNA carrier.

关 键 词：肝靶向 基因 聚乙烯亚胺 转染 乙型肝炎 

分 类 号：R346[医药卫生—基础医学]