7株TT病毒部分核苷酸及氨基酸序列分析  

Analyzing partial nucleotide and amino acid sequences of Chinese TTV isolates

作　　者：方芳[1] 王华梁[1] 王爱华[1] 邓海琳[1] 叶萍[1] 吴红萍[1] 董桂玲[1] 马丽苓[1] 吴孟超[1] 

机构地区：[1]第二军医大学附属东方肝胆外科医院实验诊断科,上海200438

出　　处：《中华实验和临床病毒学杂志》2001年第4期363-364,共2页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　了解TT病毒 (Transfusiontransmittedvirus,TTV)在我国的流行情况 ,研究我国TTV株序列特点。方法　采用半巢式聚合酶链反应 (PCR)扩增TTVDNA ,PCR阳性扩增产物直接测序。结果　我国 7例TTVDNA株部分序列与日本株相比 ,核苷酸及氨基酸同源性分别为 6 4 7%～ 98 4%及 6 2 7%～ 96 4% ;7株间的核苷酸及氨基酸同源性分别为 6 3 5 %～ 98 4%及 6 0 2 %～ 96 4% ,分别属于两种型及其中一型中的两种亚型。Objective To investigate the prevalence of transfusion transmitted virus (TTV) and its sequence characterization in china. Methods TTV DNA was amplified by Semi Nested PCR,and the positive products were sequenced directly. Results The homology of partial nucleotide and amino acid sequences of 7 Chinese TTV isolates were 64.7% 98.4% and 62.7% 96.4% respectively when compared with Japanese isolate. Among 7 Chinese TTV isolates,the homology of partial nucleotide and amino acid sequences were 63.5% 98.4% and 60 2% 96.4% respectively. They differently belong to two genotypes and two subgenotypes. Conclusion There exists TTV in China, and there might be several genotypes of Chinese TTV isolates.

关 键 词：核苷酸类 氨基酸类 序列分析 TT病毒 

分 类 号：R373[医药卫生—病原生物学]