一起引发脑膜炎流行的ECHO病毒的序列分析  

Nucelotide sequence of an ECHO virus isolated from a meningitis patient

作　　者：陈立[1] 张礼璧[1] 朱晖[1] 

机构地区：[1]中国预防医学科学院病毒学研究所诊断一室,北京100052

出　　处：《中华实验和临床病毒学杂志》2001年第4期368-372,共5页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　测定山东省招远县 1987年 2～ 8月发生的无菌性脑膜炎大流行的病因病毒之W株病毒 (Zhaoyuan W 87)的部分序列 ,分析其与其他肠道病毒的关系。方法　病毒用酚 氯仿抽提RNA ,经逆转录合成cDNA ,经聚合酶链反应 (PCR)扩增 ,产物纯化 ,采用双脱氧链末端终止法在ABI377自动核酸测序仪上测定序列。结果　W株 5′端非编码区、2C区和 3D区的部分序列长度分别为 2 44、2 75和 35 3个核苷酸 ;VP1区全基因为 876个核苷酸 ,编码 2 92个氨基酸 ,与检索到的 2株ECHO2 9核苷酸序列的同源性分别为 82 1%和 81 7% ,氨基酸序列的同源性分别为 95 1%和 94 4% ;与检索到的 1株ECHO2核苷酸序列的同源性为 6 2 3%。种系发生树分析 ,W株在单独的一个分支上 ,与 2株ECHO2 9所在分支较近 ,与ECHO2所在分支较远。结论　目前无法判定W株与ECHO2的关系 ,但可以说W株是ECHO2Objective To determine the nucleotide sequences of W strain(Zhaoyuan/W/87)isolated from a meningitis patient,and compare them with the sequences of relational enteroviruses.Methods Virion RNA was transcribed into cDNA by reverse transcriptase,cDNA was amplified by PCR,the products of PCR were purified.Then RNA sequence analysis was performed by the dideoxynucleotide chain termination method using synthetic oligodeoxynucleotide primers.Results The partial nucleotide sequences of 5′ UTR,region 2C and 3D of W strain(Zhaoyuan/W/87) were respectively 244,275 and 353 nucleotides long.The complete nucleotide sequences of region VP1 wer 876 nucleotides long coding 292 amino acids.In comparison with the VP1 sequence of two ECHO29 strains in Genbank through internet,the homogeneity of nucleotide sequence were 82.1% and 81.7%,that of amino sequence were 95.1% and 94.4%.Conclusion W strain(Zhaoyuna/W/87) was a mutation strain of ECHO29.

关 键 词：无菌性脑膜炎 肠病毒 聚合酶链反应 碱基序列 

分 类 号：R373[医药卫生—病原生物学]