甲基对硫磷ELISA分析方法的建立及初步应用  

Establishment and application of enzyme-linked immunosorbent assay for measurement of methylparathion

作　　者：王刚垛[1] 何凤生[1] 鱼涛[1] 马兆扬[1] 王伯勋 王玉萍[1] 

机构地区：[1]中国预防医学科学院劳动卫生职业病研究所,北京100050 [2]湖北省沙隆达责任有限公司职工医院

出　　处：《中国工业医学杂志》2001年第6期327-330,共4页Chinese Journal of Industrial Medicine

基　　金："九五"国家攻关课题基金资助 (96 - 90 6 - 0 4 - 1 1 )

摘　　要：目的　探讨甲基对硫磷 (M16 0 5 )简便、敏感、特异的免疫分析方法。方法　矩阵法选择好包被抗原和单克隆抗体最佳条件 ,建立M16 0 5间接竞争抑制ELISA检测法 ,并分别检测M16 0 5染毒小鼠和接触工人血清中M16 0 5含量。结果　该方法线性范围为 5～ 5 0 0ng/ml ,最低检出浓度为 5ng/ml ,组内和组间变异系数不大于 11 5 %和 14 6 % ,回收率平均为 96 2 %。M16 0 5染毒小鼠和作业工人血清中M16 0 5检出量及检出率与染毒剂量、检测时间及作业工种有关。结论　初步认为此法可用于M16 0 5接触生物标志物的检测。Objective To establish enzyme linked immunosorbent assay(ELISA)for the determination of methylparathion(M1605).Methods Indirect competitive inhibition ELISA for the determination of M1605 was developed by using anti M1605 McAb.The serum M1605 concentrations in mice treated with M1605 and in the workers exposed to M1605 were measured respectively by this method for the first time.Results The minimum detectable concentration of M1605 was 5 ng/ml,and the standard curve was linear between 5 ng/ml and 500 ng/ml,the average recovery was 96 2%,and the coefficients of variation were not over 11 5% and 14 6% for intragroup assay and inter group assay respectively.The serum M1605 concentrations in mice treated with M1605 and in workers exposed to M1605 were related with treated doses,and the time of exposure and measurement.Conclusion It seems that serum M1605 detected by immunoassay could be used as a biomarker of M1605 exposure.

关 键 词：甲基对硫磷 单克隆抗体 免疫分析 ELISA 

分 类 号：R139.3[医药卫生—劳动卫生]