湿热环境下犬肢体枪伤后外周血淋巴细胞亚群的变化  

Changes of the lymphocyte subsets expression in a canine model of gunshot wound in limbs at a hot and humid environment

作　　者：周祥吉[1] 裴国献[1] 张旭辉[1] 罗炳德[2] 

机构地区：[1]第一军医大学南方医院创伤骨科,广东广州510515 [2]第一军医大学热带病研究所,广东广州510515

出　　处：《中国工业医学杂志》2001年第6期339-341,共3页Chinese Journal of Industrial Medicine

摘　　要：目的　探讨湿热环境下机体战创伤后淋巴细胞亚群的动态变化 ,了解机体免疫功能变化的规律。方法　 2 4只南方杂种犬按外部环境随机分为常温常湿组、高温高湿组、习服组 ,并用单克隆抗体 流式细胞仪荧光抗体技术动态检测犬肢体枪伤后 ,外周血淋巴细胞表面CD4+ 、CD8+ 、CD11a+ 、CD11b+ 亚群表达结果。结果　复合因素作用下 ,淋巴细胞CD4+ 亚群表达水平 1h短暂升高 ,随后下降 ,10h后明显降低 ;CD8+ 亚群 4h后表达水平显著增高 ,相应导致CD4+ /CD8+ 比值 8h后持续降低 ;CD11a+ 亚群的降低 ( 8h后 )表明机体对感染的易感性增加 ,而CD11b+ 亚群的变化轻微 ;热习服能够增加CD4+ 的表达值 ,抑制CD8+ 大量表达。结论　CD4+ 、CD8+ 、CD11a+ 亚群以及CD4+ /CD8+ 比值的变化反映了复合因素长时间作用下能够对机体免疫功能产生抑制 ;Objective To study the changes in immunological competence of the circulating blood lymphocyte subsets in a canine model of gunshot wound in limbs at a hot and humid environment.Methods 18 Chinese southern hybrid canines are divided into 3 groups randomly according to the given environments:Group A(control),Group B(hyperthermal and humid),Group C(adaptive);the dynamic changes of the perpheral blood lymphocyte subsets including CD4 +,CD8 +,CD11a +,CD11b + expressions were detected post trauma using mAb FACS immunofluorescence technique.Results There was temporary increase(1h) and a persist decrease(10h) in CD4 + subset,while the CD8 + subset increased(4h later) that resulted in a final decrease of CD4 +/CD8 + ratio.In addition,CD11a + T cell decrease 8h later might lead to the increased susceptibility to infections.Mild change was found in CD11b + subset,the mechanism is now unclear.Adaptation training seemed to raise the level of CD4 + subsets and suppress the expression of CD8 + subset.Conclusions Changes in CD4 +,CD8 +,CD11a + lymphocyte subsets suggest that the long term effect of complex factors might suppress the immunological competence of organisms and adaptation exercise has a beneficial effect on the adverse circumstances.

关 键 词：淋巴细胞亚群 高温高湿环境 枪伤 肢体 外周血 

分 类 号：R826.5[医药卫生—临床医学]