急性梗阻性化脓性胆管炎致急性呼吸窘迫综合征大鼠肺组织内细胞因子的基因表达  

The mRNA of cytokines expression in the lungs of rats with acute severe cholangitis-induced ARDS

作　　者：崔华雷[1] 秦明放[2] 卢奕[3] 姚智[3] 鲁焕章[2] 

机构地区：[1]天津市儿童医院外科,硕士研究生300074 [2]天津市南开医院 [3]天津医科大学检验系

出　　处：《中华急诊医学杂志》2001年第5期298-299,305,共3页Chinese Journal of Emergency Medicine

基　　金：天津市科委青年科学基金资助 (94130 90 12 )

摘　　要：目的　观察急性梗阻性化脓性胆管炎大鼠ARDS时肺组织内TNF α、IL 1β的mRNA变化。方法　通过胆总管远端结扎 ,近端注入菌液并封闭的方法 ,造成大鼠AOSC模型 ,观察肺功能改变。应用原位杂交的方法检测肺组织内TNF α、IL 1β的mRNA表达。结果　模型组PaO2 /FiO2 下降 ;肺系数升高 ,肺含水量增加 ;IL 1β、TNF α的mRNA杂交信号主要位于巨噬细胞内。模型组明显高于假手术组。结论　AOSC时肺内》巨噬细胞释放大量的炎性细胞因子 ,发挥局部效应 ,导致ARDS。Objective To observe changes of the mRNA expression of IL-1β,TNF α in the lung tissue in the AOSC ARDS model.Methods AOSC model was caused by distal ligation and proximall injection bacterial liquid into the common bile duct.The functional lung change was observed.The mRNA expression of IL-1β,TNF-α in the lung tissue was observed by in situ hybridization,too.Results The results of the model group were as follow:1.PaO 2/FiO 2 decreased.2.The lung index and the water content in the lung both increased.The mRNA of IL-1β,TNF-α in the lung tissue was mainly found in the infiltrative macrophage.The expression in the model group was significant higher than that in the sham operation group.Conclusion:The lung macrophages in AOSC released a large amount of IL-1β, TNF-α.[

关 键 词：急性梗阻性化脓性胆管炎 急性呼吸窘迫综合征 细胞因子 

分 类 号：R657.45[医药卫生—外科学]