油酸钠与紫外线及维生素C灭活水泡性口炎病毒的试验观察  

EXPERIMENTAL OBSERVATION ON EFFICACY OF SODIUM OLEATE, ULTRAVIOLET RAYS AND VITAMIN C IN INACTIVATING VESICULAR STOMATITIS VIRUS

作　　者：刘蓉[1] 沈德林[1] 张文福[1] 袁庆霞[1] 钟儒波[1] 

机构地区：[1]军事医学科学院微生物流行病研究所,北京100071

出　　处：《中国消毒学杂志》2001年第3期135-140,共6页Chinese Journal of Disinfection

基　　金：国家自然科学基金(39700127)

摘　　要：采用细胞感染法，对油酸钠、短波紫外线（ＵＶＣ）、维生素Ｃ（Ｖｉｔ Ｃ）灭活水泡性口炎病毒（ＶＳＶ）的作用进行了试验观察。结果，当油酸钠含量为２．０～３．０ ｍｇ／Ｌ，作用温度为４～３７℃时，随着油酸钠含量和温度的提高，ＶＳＶ滴度下降值由 １．１７ｌｇ ＴＣＩＤ５０增至４．５０ｌｇ ＴＣＩＤ５０；而作用时间在 ０．５～２ｈ之间变化，对病毒灭活效果无明显影响。ＵＶＣ照射剂量为 ２５ ５００～２０４ ０００ Ｊ／ｍ２，可使 ＶＳＶ下降 ２．３９～５．００个对数滴度，剂量－效应关系为指数函数关系。Ｖｉｔ Ｃ对 ＶＳＶ无明显灭活作用。The efficacy of sodium oleate, short-wave ultraviolet rays (UVC) and vitamin C (vit C) in inactivating vesicular stomatitis virus (VSV) was observed experimentally by means of cell infection method. The results indicated that when 2.0~3.0 mg/L of sodium oleate was used at the temperature of 4~37℃, reduction of VSV titer increased from 1.l7lg TCID50 to 4.50 lg TCID50 with increase in concentration of sodium oleate and the temperature, while varia- tion in exposure time from 0.5 to 2 h did not influence significantly the efficacy of inactivating the virus. UVC irradiation dose of 25 500~204 000 J/m2 could reduce the VSV titer by 2.39 ~5.00 lg and the dose-effect relation was exponential function relation. Vit C had no signifi- cant inactivating effect on VSV.

关 键 词：血浆消毒 油酸钠 短波紫外线 维生素C 病毒灭活 水泡性口炎病毒 

分 类 号：R511.01[医药卫生—内科学] R187.4[医药卫生—临床医学]