平板菌落自动识别计数方法的研究  

STUDY ON METHOD OF AUTOMATIC IDENTIFICATION AND COUNTING OF BACTERIAL COLONIES ON PLATE

作　　者：杨玉琴[1] 姚楚水[1] 古希波[1] 丁兰英[1] 

机构地区：[1]军事医学科学院微生物流行病研究所,北京100071

出　　处：《中国消毒学杂志》2001年第3期141-144,共4页Chinese Journal of Disinfection

摘　　要：为判定平板菌落自动识别计数软件的功能及其影响因素，用不同菌悬液进行了活菌计数实验。结果，采取平板涂抹接种且菌落数＜３００．用该软件的程序自动计数结果，与人肉眼计数相差≤７ｃｆｕ／平板。３次自动计数结果完全一样，３人次肉眼计数结果则多不吻合。在定量杀菌试验中，当杀灭率≥７４．７６％时，用２种计数计算的结果基本一致，小于该杀灭率时两者亦相近。该自动计数法受不同微生物菌落形态、大小的影响不明显，但受琼脂倾注法接种的影响。现有程序仅适用于平板涂抹法接种所长出的菌落。In order to assess the function of the software for automatic identification and counting of the bacterial colonies on plate, experiment on viable bacteria counting was carried out us- ing different bacterial suspensions. The results indicated that when plate smearing inoculation with bacterial colonies < 300 was used, the result of automatic counting by means of this software program showed a difference of ≤7 cfu / plate as compared with the result of count- ing with naked eye. The results of triplicate automatic counting were exactly same, while the results of counting with naked eye for 3 man-times were mostly not identical. In the quantita- tive germicidal test, the results of counting by the two methods were essentially identical when the killing rate was ≥74.76% and were also similar when the killing rate was lower than 74.76%. This automatic counting method was not influenced apparently by the morphology and size of the colonies of different microorganisms, but was influenced by agar pouring in- oculation. The available program is only applicable to the bacterial colonied on the plate in- oculated by plate smearing method.

关 键 词：菌落计数 计算机程序 肉眼观察 大肠杆菌 金黄色葡萄球菌 白色念球菌 细菌芽胞 枯草杆菌黑色变种 

分 类 号：R187[医药卫生—流行病学]