铜诱导人体转铜伴侣蛋白Atox1构象变化的研究  

Study on the copper-induced conformational changes of human copper chaperone Atox1

作　　者：张雪峰[1] 贺雨虹[2] 尹珅[1] 周宏博[1] 王秀宏[1] 刘长振[1] 申峰[1] 周虹[1] 

机构地区：[1]哈尔滨医科大学生物化学教研室,黑龙江哈尔滨150086 [2]清华大学生物科学与技术系,北京100084

出　　处：《哈尔滨医科大学学报》2001年第5期324-326,共3页Journal of Harbin Medical University

摘　　要：目的　探讨铜对Atox1蛋白构象变化的影响。方法　用圆二色性光谱分析了Atox1的构象变化。结果　结合铜以后 ,Atox1无论是二级结构 ,还是三级结构都发生了变化 ,铜诱导的构象变化对于Atox1在体内发挥其作用时可能起关键作用。Objective To study the effect of copper on the conformational changes of Atox1 protein.Methods Conformational changes were analyzed by CD spectra.Results Analysis of copper -induced conformational changes of Atox1 indicated that both secondary and tertiary structure changes took place upon copper binding.These copper-induced conformational changes could play an important role in the function of Atox1 in vivo .Conclusion Copper can induce conformational changes of human copper chaperone Atox1.

关 键 词：转铜伴侣 圆二色性 构象 蛋白Atoxl 

分 类 号：Q510.1[生物学—生物化学]