爱迪诱导U251细胞凋亡及相关基因表达的实验研究  

Apoptosis and related gene expression of U251 cell line induced by Aidi in vitro

作　　者：阎志钧[1] 林志国[1] 武俏丽[2] 

机构地区：[1]哈尔滨医科大学附属第一医院X刀放疗中心 [2]哈尔滨医科大学神经外科细胞室,黑龙江哈尔滨150001

出　　处：《哈尔滨医科大学学报》2001年第5期342-344,共3页Journal of Harbin Medical University

摘　　要：目的　以人类成神经胶质瘤细胞系U2 5 1为实验对象 ,研究爱迪 (AD)诱导细胞凋亡的规律 ,并初步探讨了其内在的分子机制。方法　将爱迪分为 7 5mg/ml(A组 )、15mg/ml(B组 )、30mg/ml(C组 )、6 0mg/ml(D组 ) 4个浓度组 ,以顺铂 (DDP) 0 .4μg/ml为阳性对照组 (E组 ) ,另设一阴性对照组 (F组 ) ,观察光镜下细胞的形态变化 ,采用An nexinV PI双标记流式细胞仪凋亡检测法 ,观察爱迪诱导细胞凋亡的情况 ;采用ABC免疫组化法检查p5 3、Bcl 2和PCNA基因的表达。结果　爱迪对U2 5 1细胞具有诱导凋亡的作用 ,在 2 4h和 48h范围内 ,爱迪诱导U2 5 1细胞凋亡率与作用时间无明显关系 (P >0 .0 5 ) ,与药物浓度密切相关 (P <0 .0 1)。在爱迪浓度为 30mg/ml时 ,其凋亡率最大 ,2 4h和 48h分别为 2 0 87%和 2 0 0 9%。但爱迪浓度为 15mg/ml以上时 ,p5 3、Bcl 2、PCNA基因表达均与对照组有明显差异 ,在爱迪浓度为 30mg/ml时 ,p5 3表达高达 73 3 % ,Bcl 2降低为 2 0 9% ,随着药物浓度的增加 ,PCNA表达下降。结论　爱迪有诱导U2 5 1细胞凋亡的作用 ,其诱导凋亡依赖于p5 3基因 ,并伴有Bcl 2。Objective To study regularity of apoptosis and its gene expression induced by Aidi.Methods U251 cell was treated with Aidi that have been prepared with 7.5mg/ml(A group),15mg/ml(B group),30mg/ml(C group),60mg/ml(D group).DDP(0.4μg/ml,E group) was positive control group. U251 cell that was treated with no drugs was negative control group.We observed change of U251 cell by inverted microscopy.Apoptosis in U251 cell was detected by flow cytometer.Gene expression related with apoptosis was examined by immunohistochemical method.Results Apoptosis rate induced by Aidi was norelated with contact time of Aidi between 24 hours and 48 hours( P >0.05) and was related with concentration of Aidi( P <0.01).When the concentration of Aidi was 30mg/ml,apoptosis rate was 20.87% and 20.09% respectively at 24 hours and 48 hours.When the concentration of Aidi was over 15mg/ml,the gene expression of p53,Bcl 2 and PCNA had significant differences as compared with control group.Conclusion Aidi can induce apoptosis of U251 cell.This course depend on p53 gene and accompany a decline of Bcl 2 gene and PCNA gene.

关 键 词：爱迪 细胞凋亡 基因表达 抗癌药 

分 类 号：R979.1[医药卫生—药品] R965[医药卫生—药学]