免疫毒素BDI-1-PEA的构建及其活性测定  

The construction of immunotoxin BDI-1-PEA and the test for its cytotoxicity against human bladder carcinoma in vitro

作　　者：薛松[1] 温儒民[1] 孙晓青[1] 

机构地区：[1]徐州医学院附属医院泌尿外科,江苏徐州221002

出　　处：《徐州医学院学报》2001年第5期386-389,共4页Acta Academiae Medicinae Xuzhou

基　　金：江苏省教委自然科学基金课题 (98KJB32 0 0 0 7)

摘　　要：目的　构建新型免疫毒素BDI- 1-PEA ,并进行抗人膀胱肿瘤活性的初步研究测定。方法　应用异型双功能连接剂N -琥珀酰胺酯 3- (2 -吡啶基二硫 )丙酸 (SPDP)将抗人膀胱肿瘤单克隆抗体 (BDI- 1)与铜绿假单胞菌外毒素 (PEA)交联 ,制备出新型免疫毒素BDI- 1-PEA。应用四甲基偶氮唑盐 (MTT)法初步检测其对人膀胱肿瘤细胞系E -J的选择性杀伤活性。结果　BDI- 1-PEA结合物具有高效靶向杀伤膀胱肿瘤细胞的能力 ,优于游离PEA或PEA与BDI- 1的混合物。结论　新型免疫毒素BDI- 1-PEA有较高的选择性抗人膀胱肿瘤细胞的活性 。Objective To construct the immunotoxin BDI-1-PEA and study its cytotoxicity against human bladder carcinoma in vitro. Methods The immunotoxin BDI-1-PEA was prepared by conjugating pseudomonas exotoxin A (PEA) with monoclonal anti-bladder cancer antibody BDI-1 by using N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP). Results Study in vitro indicated that BDI-1-PEA showed stronger selective cytotoxicity against human bladder carcinoma cell line E-J than free PEA or the mixture of BDI-1 and PEA. Conclusion BDI-1-PEA conjugate is an effective immunotoxin against human bladder carcinoma and is a promising candidate for further clinical study.

关 键 词：免疫毒素 膀胱肿瘤 单克隆抗体 铜绿假单胞菌外毒素 

分 类 号：R979.5[医药卫生—药品] R967[医药卫生—药学]