异氟醚对肺泡Ⅱ型细胞表面活性物质相关蛋白A的影响  

Effect of isoflurane on pulmonary surfactant-related protein A of the alveolar type Ⅱ cells

作　　者：李永旺[1] 杨天德[1] 郝嘉[2] 刘桥义[1] 陶军[1] 史忠[1] 

机构地区：[1]第三军医大学新桥医院麻醉科,重庆市400037 [2]第三军医大学新桥医院心外科,重庆市400037

出　　处：《临床麻醉学杂志》2001年第10期556-558,共3页Journal of Clinical Anesthesiology

摘　　要：目的　通过原代培养的正常及受H2 O2 损伤的肺泡Ⅱ型上皮细胞 (ATⅡcell) ,探讨异氟醚 (Iso)对肺泡Ⅱ型上皮细胞表面活性物质相关蛋白A(SP A)的影响。方法　肺泡Ⅱ型上皮细胞培养 32小时后 ,被分为六组 :对照组 (不加任何药物 )、0 2 8mmol/LIso组、2 8mmol/LIso组、75 μmol/LH2 O2 组、75 μmol/LH2 O2 +0 2 8mmol/LIso组和 75 μmol/LH2 O2 +2 8mmol/LIso组 ,继续培养 3小时。通过酶联免疫吸附法 (ELISA)检测Ⅱ型上皮细胞内和培养液中SP A含量。结果　Iso降低正常肺泡Ⅱ型上皮细胞内和培养液中SP A含量 ;经H2 O2 作用后 ,SP A含量亦显著减少 ,异氟醚增强H2 O2 的作用。结论　Iso可降低肺泡Ⅱ型上皮细胞合成SP A的功能 ,尤其是在过氧化环境中。Objective To explore the effect of isoflurane (Iso) on pulmonary surfactant related protein(SP A) of the alveolar type Ⅱ cells cultured primary and injured by H 2O 2.Methods AT Ⅱ cells were isolated from adult rat lungs and used for the experiments after 32h in primary culture and randomised to six groups:control group,0 28mmol/L Iso group,2 8mmol/L Iso group,75μmol/L H 2O 2 group,75μmol/L H 2O 2+0 28mmol/L Iso group and 75μmol/L H 2O 2+2 8mmol/L Iso group.Each group was continuously incubated for three hours after administration of Iso or/and H 2O 2.The intracellular SP A and the SP A of cultured medium were measured with an enzyme linked immunosorbent assay(ELISA).Results Iso significantly decreased SP A content of cultured medium and the intracellular,and augumented the decrease of SP A content induced by H 2O 2 in a dose dependent manner.Conclusion Isoflurane may decrease SP A synthesis of the alveolar type Ⅱ cells in vitro,and aggravate the damage of the alveolar tpye Ⅱ cells especially under peroxidation condition . [

关 键 词：异氟醚 表面活性物质相关蛋白A 肺泡Ⅱ型上皮细胞 

分 类 号：R614.1[医药卫生—麻醉学]