一种简易液相色谱柱切换法测定血清中丝裂霉素的含量  

A Simple Column Switching Method for the Determination of Mitomycin in Serum by Liquid Chromatography

作　　者：陈子易[1] 何宝俊 王晓辉[1] 王瑛珏[1] 

机构地区：[1]中国医科大学化学检测中心实验室,沈阳110001

出　　处：《色谱》1991年第5期320-322,共3页Chinese Journal of Chromatography

摘　　要：丝裂霉素是目前广泛使用的一种抗癌药物,临床有多种给药方式,其疗效不尽相同,因此测定丝裂霉素在血中的浓度对了解某种给药方式的疗效无疑是很有意义的。目前丝裂霉素血药浓度的测定方法,一般采用Pinedo所介绍的高效液相色谱法,该法的血样前处理复杂。A Simple column switching method of liquid chromatography is presented for the determina- tion of Mitomycin in serum.Two Sep-pak C18 Cartridge small columns were used for on-line pretreatment of sample and its analysis respectively.The eluants were detected by UVabsorption at 365nm.The calibration curve was linear within the range of 20- 450ng/ ml.The average recovery was 99.56%.

关 键 词：血清 丝裂霉素 高效液相色谱 

分 类 号：R446.119[医药卫生—诊断学]