细胞因子组合对小鼠骨髓单核细胞的体外扩增作用  

Effects of a combined cytokines on ex vivo expansion of BMMNC

作　　者：孔佩艳[1] 郭朝华[2] 罗成基[2] 周燕虹[2] 邹仲敏[2] 施炎[2] 

机构地区：[1]第三军医大学附属新桥医院血液内科,重庆400037 [2]第三军医大学预防医学系防原医学教研室,全军复合伤研究所,重庆400038

出　　处：《第三军医大学学报》2001年第10期1167-1169,共3页Journal of Third Military Medical University

基　　金：全军"九五"指令性攻关课题 ( 96L0 4 5 )

摘　　要：目的　了解细胞因子组合对小鼠骨髓单个核细胞的体外扩增作用。方法　采用SCF、IL 3、IL 6、GM CSF、G CSF和EPO等 6种细胞因子组合在体外扩增培养小鼠骨髓单个核细胞 5d后 ,收集培养细胞作体外半固体培养计数各不同阶段祖细胞数 ,并计数细胞总数 ,与未经扩增培养的细胞作比较以判断其扩增情况。结果　有核细胞总数扩增不显著 ,为 (1 .3 60± 0 .1 2 6)倍 ,但造血祖细胞却获得了明显扩增 ,其中CFU E达培养扩增前的 (7.0 4± 1 .2 6)倍 ,BFU E为 (4 .74± 1 .0 4)倍 ,CFU GM和CFU Cs则分别为 (1 .78± 0 .47)和 (1 .74± 0 .48)倍。由此可见 ,在此培养体系中以红系祖细胞的扩增最为显著。结论　小鼠骨髓单个核细胞在单纯细胞因子存在的扩增体系中具有一定的扩增其细胞总数和集落形成祖细胞数的能力 ,为进一步作体内实验判断其造血重建的功能奠定了理论基础。Objective To study the effects of combined application of cytokines including SCF,IL 3,IL 6,GM CSF,G CSF and EPO on ex vivo hematopoietic progenitor expansion. Methods Using the techniques of ex vivo bone marrow mononuclear cells expansion, semisolid culture of colony forming unit progenitor cells. Results While adding the cytokines to the cultural systems of ex vivo expansion of BMMNC for 5 days, the number of total cells was (1.360±0.126) times, CFU E,BFU E,CFU GM and CFU Cs were increased notably than those of the uncultured control group. Conclusion The results indicated that the ex vivo expansion of BMMNC in the system containing combined cytokines could obviously promote the expansion of the hematopoietic progenitors, and this meight be the basis for the further study of the effects of ex vivo expanding BMMNC on hematopoietic constitution in vivo.

关 键 词：细胞因子 骨髓单个核细胞 体外扩增作用 

分 类 号：R392.11[医药卫生—免疫学] R329.25[医药卫生—基础医学]