HCV体外感染正常成人肝细胞的研究  

Study on the infection of nomal adult hepatocytes with HCV in vitro

作　　者：马巧玉[1] 郝飞[1] 王宇明[1] 

机构地区：[1]第三军医大学附属西南医院全军感染病学研究所,重庆400038

出　　处：《第三军医大学学报》2001年第10期1216-1218,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目 ( 39770 6 82 )

摘　　要：目的　为进一步研究丙型肝炎免疫发病机制、临床治疗和疫苗研制提供最接近自然感染状态的理想细胞模型。方法体外二步灌流法分离正常成人肝细胞并维持培养 1月以上 ,丙型肝炎患者血清感染上述肝细胞并用免疫组化和逆转录 聚合酶链反应法 (RT PCR法 )检测被感染肝细胞HCV抗原表达及HCVRNA。结果　正常成人肝细胞可在体外存活 1月以上 ,加丙肝患者血清4～ 3 6d后免疫组化法可检测到肝细胞内HCVNS3、NS5抗原表达 ,感染后 2～ 3 6d ,RT PCR法可在感染肝细胞培养上清和细胞悬液中间断检测出HCVRNA。结论　正常成人肝细胞可在体外存活 1月余并被HCV感染者血清感染。Objective To provide a better cell model of closely nature infectious state for further understanding the immunopathogenesis, clinical treatment and vaccine development of hepatitis C. Methods Normal adult hepatocytes were isolated according to the two step perfusion method and cultured in vitro more than 1 month. The hepatocytes were then infected with serum of patients with hepatitis C. The expression of HCV antigen and HCV RNA in cells were detected with immunohistochemical method and RT PCR. Results Normal adult hepatocytes surrived more than 1 month in vitro . In this experiment the normal adult hepatocytes could be infected by serum containing HCV from patients with hepatitis C. The expression of HCV NS3, NS5 antigen in cells could be detected by immunohistochemical method, HCV RNA was discontinuously detected in cells and supernatant with RT PCR. Conclusion Normal adult hepatocytes may survive more than one month in vitro and can be infected by serum of patients with hepatitis C.

关 键 词：成人 肝细胞 细胞模型 丙型肝炎病毒 

分 类 号：R373.21[医药卫生—病原生物学]