湿热环境下犬肢体火器伤后中性粒细胞CD11a、CD11b表达的变化  

Changes of CD11a and CD11b expression in the neutrophils of dogs after gunshot wound in the limb in hot and humid environment

作　　者：周祥吉[1] 裴国献[1] 张旭辉[1] 罗炳德 

机构地区：[1]第一军医大学南方医院创伤骨科,广东广州510515 [2]第一军医大学高温研究室,广东广州510515

出　　处：《第一军医大学学报》2001年第10期737-739,共3页Journal of First Military Medical University

基　　金：军队九五医药卫生杰出中青年基金(98J004)

摘　　要：目的探讨湿热环境下犬肢体火器伤后外周血中性粒细胞CD11a、CD11b表达的变化规律。方法建立湿热环境下犬肢体枪伤模型,用流式细胞术测定不同时间点外周血中性粒细胞表面CD11a、CD11b的表达情况,并且对创伤区组织进行细菌计数。结果常温常湿组CD11a、CD11b在致伤后即刻以及伤后4h显著增高;与常温常湿组比较,高温高湿组第2峰表达水平显著增高,但下降时间提前;犬习服后可以降低第2峰的高度,并且延长其平台期。细菌记数与两者表达在时间上有相关性。结论CD11a、CD11b表达水平及其变化能够作为疾病不同进展阶段的标志,两者介导了中性粒细胞对伤道外组织损害的炎症过程。Objective To investigatethechangesof CD11aandCD11blevelsinperipheralbloodneutrophils(PMNs)of dogswithgunshotwoundinthelimbinhotandhumidenvironment.Methods Caninemodelsof gunshotwoundinthelimb were establishedand subsequentlykeptin hot and humidenvironment. The dynamicchangesof CD11aand CD11b expressionin PMNs afterinjurywere measuredby meansof mAb-FACS-immunofluorescencetechniqueand bacterial quantificationin the woundperformed.Results The expressionsof CD11aand CD11bin the controlgroup(normal environment)increasedimmediatelyafterinjury,presentinga secondpeakat4h aftertheinjury.Thesecondpeakwas also observedin hot and humidgroupafter4h and was higherbutof shorterdurationcomparedwiththosein controlgroup.Thermo-adaptivetrainingremarkablyprolongedtheplatformstageof thesecondpeakandlowereditslevel.Thequantityof thebacteriainthewoundwascorrelatedwiththeexpressionsof CD11aandCD11b.Conclusions Thelevelsandchangesof CD11aandCD11bmarkthecoursesof diseasedevelopment,andtheirexcessiveexpressioninducesneutrophil-mediated inflammatoryresponseleadingto damageto thenormaltissues.

关 键 词：高温 高湿 创伤 枪击 四肢 中性粒细胞 粘附分子 

分 类 号：R392.3[医药卫生—免疫学]