益气通络丹抗缺氧再给氧心肌细胞凋亡的实验研究  

Yiqitongluo pellet inhibits cardiomyocyte apoptosis induced by reoxygenation following hypoxia:an experimental observation

作　　者：沈剑刚[1] 佟丽[1] 邱幸生[1] 曲宏达[1] 陈育尧[1] 

机构地区：[1]第一军医大学中医系,广东广州510515

出　　处：《第一军医大学学报》2001年第10期743-745,共3页Journal of First Military Medical University

基　　金：国家自然科学基金(39300178);广东省自然科学基金(95056719)

摘　　要：目的观察含益气通络丹的大鼠血清对缺氧24h再给氧4h后新生乳鼠心肌细胞凋亡的抑制作用及其对心肌细胞乳酸脱氢酶(LDH)释放、一氧化氮(NO)生成和硫代巴比妥酸反应物质(TBARS)的影响。方法采用AnnexinV-PI双标记方法以流式细胞仪和ELISA法检测细胞凋亡,以紫外分光光度法测定LDH释放水平,采用改良Yu方法测定NO浓度,用Ohkawa法测定TBARS含量。结果心肌细胞缺氧再给氧后NO、TBARS水平升高,LDH释放增加。含益气通络丹的血清能显著抑制心肌细胞凋亡,抑制LDH释放,降低培养细胞上清液中NO和TBARS水平,其效应呈剂量依赖关系。结论益气通络丹具有抗缺氧再给氧心肌细胞凋亡作用,其机制与清除氧自由基和NO特性有关。Objective To investigatethe inhibitoryeffectof Yiqitongluopelleton culturedneonatalrat cardiomyocyte apoptosisinducedby reoxygenationwithprecedinghypoxiaandobservethechangesin thelevelsof lactabledehydrogenase,nitricoxide(NO)andthiobarbituricacid-reactivesubstances(TBARS)in theinoculum.Methods The neonatalrat cardiomyocyteswereobtainedto establishmodelsof reoxygenationfollowinghypoxia,andsubsequentlytreatedwiththe serumisolatedfrom Yiqitongluopellet-fedWistarratsadministeredbeforeannexinV-PIlabeling.Flowcytometryand enzyme-linkedimmunosorbentassaywereperformedfor cellularapoptosisassessment.The concentrationof LDHin the inoculumwasmeasuredwithspectrophotometer,andimprovedYu methodandOhkawamethodwereemployedto determine NO and TBARSlevels,respectively.Results Yiqishuluopelletratserumsignificantlyinhibitedthecardiomyoctesfrom apoptosisinducedby reoxygenationwithprecedinghypoxia,and the elevatedNO,LDH and TBARSlevelsdue to reoxygenationintheinoculumwerebroughtdownby themedication-containingserum.Conclusion Yiqitongluocaninhibite cardiomyocyteapoptosisinducedby reoxygenationfollowinghypoxiaby themechanisminvolvingtheeliminationof oxygen freeradicalsandNOproductionregulation.

关 键 词：供氧 通络 脱噬作用 心肌再灌注 益气通络丹 

分 类 号：R285.5[医药卫生—中药学]