单细胞凝胶电泳图像分析系统的研制及应用  

Design and application of image analysis system to single cell gel electrophoresis

作　　者：朱志良[1] 庄志雄[2] 黄钰[2] 籍涛[3] 

机构地区：[1]深圳市宝安区卫生防疫站科教科,518101 [2]中国预防医学科学院深圳预防医学中心毒理研究室 [3]中山医科大学生物医学工程研究所

出　　处：《中华劳动卫生职业病杂志》2001年第4期298-300,F004,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的　编制单细胞凝胶电泳 (SCGE)图像分析系统 (IMI 1.0 )。方法　利用Dephi 5作为工具 ,设计的分析指标有长度、强度、矩类以及头尾DNA含量比等 4类指标。结果　应用该系统检测了谷胱甘肽(GSH)在H2 O2 致DNA损伤中的作用 ,尾长、尾重心、Olive尾矩、尾惯量、尾分布矩、尾DNA含量 ( %)以及尾 /头比等指标与H2 O2 浓度存在明显的剂量 -效应关系。Olive尾矩与H2 O2 浓度的剂量 -效应关系线性良好 ,是SCGE检测DNA损伤的最适指标。 10 μmol/LGSH能抑制H2 O2 对DNA的断裂损伤。结论　IMI 1.0操作快捷方便 ,设计的指标能满足SCGE图像分析的要求。Objective To design image analysis system for single cell gel electrophoresis(SCGE). Methods The image analysis soft was designed with Dephi 5.Inhibition of GSH on H 2O 2-induced breakage of single strand DNA in peripheral lymphocyte was detected. Results There were dose-response relationship between H 2O 2 and indexes including length of tail,tail mean,tail Olive moment,tail inertia,tail distributed moment,tail DNA percentage and ratio of tail/head.10 μmol/L of GSH could inhibit H 2O 2-induced breakage of DNA. Conclusion The image analysis software was a good tool for SCGE comet analysis.

关 键 词：单细胞凝胶电泳 图像分析 H2O GSH 谷胱甘肽 DNA损伤 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]