对苯二甲酸诱导大鼠膀胱癌的分子病理学研究  

Study on the molecular pathology of terephthalic acid-induced urinary bladder cancer

作　　者：漆少廷 王心如[1] 徐锡坤[1] 向全永[1] 许娟华[1] 汪建平[1] 

机构地区：[1]南京医科大学应用毒理研究所,210029

出　　处：《中华劳动卫生职业病杂志》2001年第4期301-303,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：卫生部自然科学基金资助项目 ( 3 92 10 9) ;江苏省科委自然科学基金资助项目 ( 92 0 33)

摘　　要：目的　研究对苯二甲酸 (TPA)诱导大鼠膀胱肿瘤的分子病理学改变。方法　用喂饲法以5 0 0 0、5 0 0、5 0mg/kgTPA分别对SD大鼠连续染毒 90d。对大鼠进行普通病理学检查。用免疫组化法检测大鼠膀胱p5 3和增殖细胞核抗原 (PCNA)的表达情况。结果　 5 0 0 0mg/kgTPA染毒大鼠膀胱移行细胞癌发生率为 2 3 .5 %( 4 / 17) ,不典型增生率为 2 9.4 %( 5 / 17) ,单纯增生率为 4 1.2 %( 7/ 17) ;5 0 0mg/kgTPA染毒大鼠不典型增生率为 5 5 .6%( 10 / 18) ,单纯增生率为 11.1%( 2 / 18) ;5 0mg/kgTPA染毒大鼠单纯增生率为 5 8.8%( 10 / 17) ;对照组大鼠单纯增生率为 5 .6%( 1/ 18)。TPA染毒大鼠PCNA的表达与病理改变呈等级正相关 (rs=0 .79) ,与染毒剂量呈直线相关 [P <0 .0 1,rate=0 .183+0 .0 5 7×dose(g) ];P5 3蛋白在各组大鼠中均未见明显表达。结论　TPA可诱导大鼠PCNA的过度表达 ,与膀胱移行细胞癌的发生发展有一定的关系 ,p5 3基因的表达与膀胱癌关系不明显。Objective To study the pathological molecular mechanism of terephthalic acid(TPA)-induced urinary bladder cancer. Methods SD rats were administer TPA by oral feeding at dose of 5 000,500,50 mg·kg -1·d -1 for 90 consecutively days.Control animals received normal feeding.Pathologic examinations were made on the bladders of experimental rats.The expression of proliferating cell nuclear antigens(PCNA) and p53 in the paraffin-embedded bladders were detected. Results There were 4 cases of bladder transitional cell cancer (4/17) and 5 cases of atypical hyperplasia(5/17) as well as 7 cases of simple hyperplasia(5/17) in 5 000 mg/kg group,10 cases of atypical hyperplasia(10/18) and 5 cases of simple hyperplasia(10/18)in 500 mg/kg group,10(10/17) and 1 cases(1/18)of simple hyperplasia in 50 mg/kg and control groups respectively.There was positive rank correlation between positive rate of PCNA and pathologic changes(P<0.01, r s=0.79),and linear correlation between the rate and dose[P<0.01,[WT6]rate= 0.183+0.057×dose(g) .p5 3wasnotexpressedmarkedlyinallthe animals.Conclusion TPAcouldpromotePCNAexpressionandthismaybethemechanismofTPAinducedblad dercancer,whilep5 3expressionwasnotmarkedlyrelatedtobladdercancer.

关 键 词：对苯二甲酸 膀胱癌 增殖细胞核抗原 p53 分子病理学 

分 类 号：R737.14[医药卫生—肿瘤]