水分胁迫下内生真菌感染对黑麦草叶内游离脯氨酸和脱落酸含量的影响  

Effect of endophyte infection on the concentrations of free proline and ABA of leaves of Lolium perenne L. under water stress

作　　者：陈世苹[1] 高玉葆[1] 梁宇[1] 任安芝[1] 

机构地区：[1]南开大学生命科学学院,天津300071

出　　处：《生态学报》2001年第12期1964-1972,共9页Acta Ecologica Sinica

基　　金：国家重点基础研究发展规划项目 (编号 G2 0 0 0 0 1 86 0 1 );国家自然科学基金 (编号 39870 1 4 2 )资助项目

摘　　要：以黑麦草为实验对象 ,研究了干旱胁迫条件下内生真菌感染对植株叶片含水量和叶内游离脯氨酸含量的影响 ,同时对渗透胁迫条件下植株叶内 ABA含量的变化进行了分析。结果表明 :1内生真菌的感染有助于使叶片保持较高的含水量 ;2在两种形式的水分胁迫下 ,前期至中期高感染种群的叶片游离脯氨酸含量低于低感染种群 ,而在末期则有高出低感染种群的趋势 ;3内生真菌感染对黑麦草叶内Endophytes or Endophytic fungi are a kind of fungi that live inside plant. They widely exist in higher plants, particularly in grasses. The fungi are members of class Ascomycetes, family Clavicipitaceae, tribe Balansiae, including some imperfect fungi that are in close relationship with tribe Balansiae, such as fungi of Acremonium. It has been well documented that endophytes and their host plants are mutually symbiotic. On one hand, plants provide photosynthates for the fungi; on the other hand, endophytes may enhance the host's growth and resistance to abiotic stresses (such as drought, high temperature) and biotic stresses (such as herbivore graze).Although the mechanisms of how endophytes enhance the drought resistance of host plant are still unknown, it is clear that endophytes benefit their host through affecting its substance metabolism, or through producing physiologically activated substances (i.e. alkaloid, hormone). Some researchers have found that the infected plants have greater ability of osmotic regulation, so as to maintain a higher turgor pressure and thus keep growing under water stress. Most of these studies, however, were focused on polyols. Two important molecules in osmotic regulation, proline and ABA, have seldom been mentioned. A comparative study of HI (highly-infected) and LI (low-infected) populations were conducted under two different water stress conditions. Physiological characteristics related to drought resistance of plants, including leaf water content (LWC), free proline content and abscisic acid (ABA) content, were determined.. Seeds of Lolium perenne\% cv Pinnacle (from Oregon Seed Company, USA) were treated in two different ways: some were placed on moist filter paper and germinated under room temperature to get HI plants, others were exposed to heat treatment in bath (43℃15min, then 57℃ 35min) and then placed on moist filter paper to get LI plants. The experiment was conducted on the campus of Nankai University, Tianjin, China. There were 18 plots, 40cm×50cm in size

关 键 词：水分胁迫 内生真菌 黑麦草 游离脯氨酸 脱落酸 

分 类 号：S543.6[农业科学—作物学]