小鼠杂交瘤细胞在双口滚动培养瓶中的生长及抗体分泌  

Growth and Antibody Production of Resultant Hybridoma Cells in a Double-mouthed Roller

作　　者：王彦[1] 焦鸿丽[1] 张锦珠[1] 赫荣乔[1] 

机构地区：[1]中国科学院生物物理研究所视觉信息加工开放实验室,北京100101

出　　处：《生物化学与生物物理进展》2001年第5期744-747,共4页Progress In Biochemistry and Biophysics

基　　金：国家 8 63 2高技术航天领域项目 (863 2 7 2 16)~~

摘　　要：研制了一种新的滚动式细胞培养装置 (rollingculturesystem)和双口滚动瓶 (double mouthedroller) .利用分泌抗人绒毛膜促性腺激素 (hCG)单克隆抗体的小鼠杂交瘤细胞作为检验材料 ,对培养在双口滚动培养装置及常规T形瓶中的细胞生长和单抗分泌进行了比较 .在滚动培养装置中 (转速 2～ 10r/min)培养的细胞生长和抗体分泌皆增加 30 %以上 .不同浓度的血清对细胞生长和抗体产量有一定影响 ,含 5 %血清的培养液中生长的杂交瘤细胞单抗产量最高 ;A new rolling culture system with double-mouthed roller was manufactured. Hybridoma, which produces anti-hCG monoclonal antibody (mAb), was employed in the double-mouthed roller, in which growth and antibody yield of the cells were compared with those cultivated in T flask. The results showed that with an increase in growth of the cells cultured in the roller by 1.4 similar to 1.8 folds, the yield of the antibody increased by 1.3 folds of those statically cultured in T flask. Addition of gelatin in culture media could further improve both yield of the antibody and growth of the hybridoma cells.

关 键 词：杂交瘤 单抗分泌 细胞培养 双口滚动瓶 生长 抗体 

分 类 号：R392－33[医药卫生—免疫学]