部分小檗科植物的RAPD分析  

RAPD ANALYSIS ON SOME SPECIES OF BERBERRIDACEAE

作　　者：王艇[1] 苏应娟[1] 朱建明[1] 范国宽[1] 陈瑾[1] 

机构地区：[1]中山大学生命科学学院,广州510275

出　　处：《植物研究》2001年第3期428-431,共4页Bulletin of Botanical Research

基　　金：国家自然科学基金 ( 395 0 0 0 13;3970 0 117);广东省自然科学基金 ( 95 0 0 99;970 176 )资助项目

摘　　要：利用随机扩增多态性DNA(RAPD)技术分析了部分小檗科 (Berberidaceae) 5个属 6种植物 :猫儿刺 (BerberisjulianaeSchneid .) ,日本绿叶小檗 (BerberisthunbergiiDC .) ,阔叶十大功劳(Mahoniabealei (Fort.)Carr.) ,南天竹 (NandinadomesticaThunb .) ,淫羊藿 (Epimediumsagit tatum(S .etZ .)Maxim .)和八角莲 (Dysosmaversipellis (Hance)M .Cheng .)。经筛选Sangon公司的 6 0个引物 ,其中 2 9个引物的谱带清晰并呈多态性。采用UPGMA法对求出的遗传距离进行聚类分析 ,结果显示 :在小檗科内建立十大功劳属 (Mahonia)和南天竹属 (Nandina)The fresh leaves of Berberidaceae were collected as materials including 5 genera and 6 species: Berberis julianae Schneid., Berberis thunbergii DC., Mahonia bealei (Fort.)carr.,Nandina domesrtica Thunb., Epimedium sagittatum(S.et Z.)Maxim., Dysosma versipellis (Hance)M.Cheng.. The total DNA were isolated by improved CTAB method. 60 random primers produced by Sangon were screened and 29 could amplify good RAPD Products. The dendrogram was constructed from genetic distances through UPGMA method, and the phylogeny of Berberidaceae was discussed based on it.

关 键 词：小檗科 RAPD 系统发育 植物 

分 类 号：Q949.746.8[生物学—植物学]