生物信息学技术克隆并分析新基因STRF7  

Molecular cloning and analysis of novel STRF7 gene by bioinformatics strategy

作　　者：黄耀江[1] 黎燕[2] 王关林[3] 沈倍奋[2] 

机构地区：[1]大连理工大学生物化学工程研究所,辽宁大连116012 [2]北京基础医学研究所,北京100850 [3]辽宁师范大学生物工程研究所,辽宁大连116029

出　　处：《大连理工大学学报》2001年第4期446-450,共5页Journal of Dalian University of Technology

基　　金：国家杰出青年科学基金资助项目 (3992 5 0 19)

摘　　要：为进一步研究信号转导相关的新基因片段 BE644 2 50 ,采用生物信息学方法克隆其全长 c DNA,并分析了其 ORF、电子表达谱、染色体定位等 ;之后对全长序列进行了实验验证 .电子延伸 ( contig)获得了 72 9bp的延伸产物 ,含一个典型的 74 aa的 ORF,命名为 STRF7;与已知蛋白无明显同源性 ,部分地相似于人的同源框蛋白 CDX-4和酵母的转录调节子 ADR6,属一新发现的基因 ;RT-PCR从 IL-6刺激后的 U937中克隆了 STRF7基因 ,其序列与电子延伸结果完全一致 ;进一步的分析显示 STRF7在多种组织中表达并定位于第 6号染色体上 .上述结果显示 ,STRF7是一个新基因 ,编码含 74 aa的蛋白 。In order to study novel cDNA fragments BE644250, the authors analyzed full length cDNA sequences, open reading frame (ORF), electronic expression pattern and position on chromosome by bioinformatics methods and laboratory bench work. In the results, 729 bp contig products were obtained by contig, named as STRF7, the open reading frame of STRF7 gene encodes a putative protein containing 74 amino acid residues. Blast against the current GenBank DNA and protein sequence database did not reveal significant homology with any known proteins, partial similarity to human homeobox protein CDX 4 and yeast transcription regulatory protein ADR6. The STRF7 full length cDNA sequence has been cloned from IL 6 induced U937 cells by RT PCR. In addition, novel genes STRF7 expressed in many tissues by electronic expression pattern analysis and mapped to human chromosome 6. These results suggest that STRF7 may be transcription factor and novel STRF7 gene which encodes a protein containing 74 amino acid residues.

关 键 词：基因克隆 生物信息学 STRF7基因 CDNA序列分析 表达谱 染色体定位 

分 类 号：Q785[生物学—分子生物学]