番茄耐盐愈伤组织的筛选及显微结构观察  

Selection of NaCl tolerant calli and observation of microstructure

作　　者：戴伟民[1] 赵艳[1] 蔡润[1] 潘俊松[1] 何欢乐[1] 陆雅君[1] 

机构地区：[1]上海交通大学植物科学系,上海201101

出　　处：《上海交通大学学报（农业科学版）》2001年第2期112-116,共5页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：上海市科委资助项目 (983 913 0 13 )

摘　　要：以番茄下胚轴为外植体诱导愈伤组织 ,转入含 10 g LNaCl的筛选培养基上培养 ,经过 3代高盐直接筛选 ,获得耐盐愈伤组织 ,进行电镜观察 ,结果表明 ,存活下来的耐盐绿色愈伤组织细胞及细胞器 (与对照相比 )形态结构正常 ,代谢活动旺盛 。The calli produced from hypocotyl of tomato,that had been maintained for 60d, were transferred to media supplemented with NaCl 10g/L for callus selection.NaCl tolerant calli were obtained by three generations of selections.On medium containing NaCl 10g/L,cells and their organelleles in NaCl tolerant calli had normal morphology and structure,and vigorous metabolism;but in untolerant calli,the majority of cells turned towards dying.

关 键 词：番茄 耐盐愈伤组织 细胞显微结构 

分 类 号：S641.2[农业科学—蔬菜学]