用DNA斑点杂交法检测对虾及其饵料和环境生物携带白斑综合症病毒状况的调查  

Investigation and study on carrying status of White Spot Syndrome Virus(WSSV) in shrimps and other aquatic animals by dot-blot hybridization

作　　者：宋晓玲[1] 史成银[1] 黄倢[1] 张立敬[1] 

机构地区：[1]中国水产科学研究院黄海水产研究所,山东青岛266071

出　　处：《中国水产科学》2001年第4期36-40,共5页Journal of Fishery Sciences of China

基　　金："九五"国家重点科技攻关计划项目 (96-0 0 5 -0 3 -0 1);国家重点基础研究规划资助项目 (G19990 12 0 0 2 )

摘　　要：于 1996～ 1998年采用DNA斑点杂交法 ,完成了共计 836份养殖对虾 ,2 13份对虾饵料和环境生物样品的检测。结果显示 ,对虾在每年的 4～ 10月都可检测出白斑综合症病毒 (WSSV) ,6月出现最高峰 ,阳性检出率达到 4 2 .6 % ;其次 ,为 7月和 5月 ,分别达 31.6 %和 2 4 .5 %。在对虾不同生长阶段 (包括亲虾、各期幼体、仔虾、幼虾及成虾 )均可检出WSSV ,其中以仔虾的带毒率最高 ,达 36 .4 % ,其次为糠虾幼体 ,达 34.6 %。体长范围 1.1～ 2 .0cm的仔虾阳性率最高 ,其次体长为 5 .1～ 6 .0cm的幼虾样品。检测的中国对虾样品(6 2 5份 )带毒率为 16 .8% ,日本对虾样品 (2 11份 )带毒率为 4 5 .0 %。对虾主要饵料及环境生物类群包括鱼类、虾类、蟹类、桡足类、端足类、沙蚕、卤虫均可检出WSSV ,其中小型虾类、端足类、桡足类、蟹类样品的WSSV检出率均高于 2 0 %。About 836 samples of Penaeus chinensis (625 inds), P.Japonicus(211 inds) and 213 other aquatic animal samples were collected from the cultural farms in Liaoning, Hebei, Tianjin, Shandong, Jiangsu and Zhejiang Provinces during 1996～1998. The method of dot blot hybridization was employed and a digoxigenin labelled probe was used to detect the White Spot Syndrome Virus (WSSV) in the samples. The results show that the WSSV can be detected in the shrimp samples collected from April to November each year and the detectable peak appears in June that the positive rate gets to 42.6% and the secondary peak is in July and May when the positive rates get to 31.6% and 24.5%, respectively. Otherwise, WSSV can be detected at different stages of the shrimps, including the parents, ovum, larvae, postlarvae, mysis, juveniles and adults, among which the positive rate is the highest in the postlarvae (36.4%) and the second highest in the mysis (34.6%). On the basis of body length of shrimp, the positive rate is the highest in the group of body length 1.1～2.0 cm and the second highest in the group of 5.1～6.0 cm. The positive rate is 16.8% for P. Chinensis and 45.0% for P. japonicus. Among the other animal samples, WSSV can be detected in artemia, nereidae, copepoda, amphipoda, non penaeid shrimp, crabs and fishes and the positive rate of WSSV is more than 20% in copepoda, amphipoioda, amphipoda, non penaeid shrimp and crabs.

关 键 词：中国对虾 日本对虾 铒料 环境生物 白斑综合症病毒 DNA斑点杂交 

分 类 号：S945.46[农业科学—水产养殖]