沙柳等位酶分析  

THE ANALYSIS OF THE ALLELIC ENZYME OF Salix psammophila

作　　者：乌云塔娜[1] 张胜利[1] 秦月明[1] 安守芹[1] 

机构地区：[1]内蒙古农业大学生态环境学院,呼和浩特010019

出　　处：《内蒙古农业大学学报（自然科学版）》2001年第3期74-79,共6页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

摘　　要：本研究运用等位酶标记基因 ,通过 4种酶系统对 92个沙柳无性系进行优良基因型筛选 ,其中过氧化物酶和天冬氨酸转氨酶采用凝胶系统 分离 ,酯酶和己糖激酶采用凝胶系统 分离。研究结果表明 :4种酶系统至少有10个基因位点所控制 ;各位点表现高度杂合性 ;其中至少 3个位点有多态性 ;Pod- 2、Pod- 3的重复位点和 Hex-2位点 ;筛选出 Pod- 2 b基因和 Hex- 2 d基因为沙柳高生长性状的标记基因 ,其树高数量性状为同时含有 Hex- 2 d和 Pod- 2 b >只含有 Hex- 2 d >只含有 Pod- 2 b >不含有 Pod- 2 b和 Hex- 2 d,筛选出 XIV- 0 8等 7个优良无性系。In the article the gene of Salix psammophila was marked by allelic enzyme,and the excellent clones were select from ninetyjjj-two clones by four enzyme system.Among them peroxidase and aspartate transaminase were separated by Ⅰ system of gelatin,and Esterase and hexokinase by Ⅱ system of gelatin.The result showed that four enxyme system were controlled by ten gene site in at least,and every site showed deeply heterozygosity.Three sites at least have polymorphism involving Pod-2,pod-3 repeat site and Hex-2 site,etc..Pod-2 b and Hex-2 d were selected and regarded as marker gene of the high-growing of Salix psammophila.Its height quantity character were order from high to low:hex-2 d and Pod-2 b,Hex-2 d,Pod-2 b.The lowest height quantity character hasn't Hex-2 d and Pod-2 b.Seven excellent clones involving ⅪⅤ-08,etc.were selected.

关 键 词：沙柳 等位酶 标记基因 

分 类 号：Q943.2[生物学—植物学]