猪肌生成抑制素基因的克隆和序列测定  

Cloning and Sequence Analysis of Porcine Myostatin Gene

作　　者：欧阳红生[1] 孙燕[1] 张永亮[1] 李慎涛[1] 张锐[1] 廖晓萍[1] 张玉静[1] 赵建军[1] 赵凤云[1] 

机构地区：[1]解放军军需大学基础部,吉林长春130062

出　　处：《中国兽医学报》2001年第5期479-481,共3页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目 ( 30 0 70 5 6 3);军队医药卫生基金资助项目 ( 98Q 0 79)

摘　　要：根据文献报道的猪肌生成抑制素基因 c DNA序列 ,分别从第 1、第 2和第 3外显子中设计引物 ,以大约克猪染色体 DNA为模板 ,分段扩增出含内含子和外显子的序列。将所得各序列回收、克隆到 p GEM-easy T载体中 ,然后进行序列测定。合并所有测得的猪肌生成抑制素基因序列 ,共获得 6 kb连续序列。分析结果表明 ,猪肌生成抑制素基因第 1内含子长 1 80 9bp,第 2外显子长 374 bp,第 2内含子长 1 978bp。所测得的外显子序列与已发表的 c DNA序列同源分析比较 ,没有出现引起氨基酸改变的突变。表明瘦肉型猪 (大约克猪 )肌生成抑制素基因仍很完整 。Ten oligonucleotide primers were designed and synthesized according to the nucleotide sequence of porcine myostatin c DNA. Using the primers,the whole gene of porcine myostatin was amplified by PCR from porcine DNA which extracted from pig blood. Four fragments ( the upstream flanking region-the exon 1 ,the intron 1 -exon 2 ,the in- tron 2 -exon 3,and the exon 3) ,prepared from PCR products,were cloned into the p GEM-T Easy Vector( Promega) . The nucleotide sequences of all four fragments from the recombinant plasmid were determined. The 6 kb nucleotide se- quence of the whole porcine myostatin gene was obtained. The result showed that the intron 1 and the intron 2 of porcine myostatin gene must be 1 80 9bp and 1 978bp,rsepectively.

关 键 词：猪 肌生成抑制素 基因 克隆 序列 

分 类 号：S828.2[农业科学—畜牧学]