云南普通野生稻和疣粒野生稻组织培养的研究  

Regenerating green plants from calli of Yunnan wild rice, Oryza rufipogon Griff and Oryza meyeriana Baill

作　　者：张尧忠[1] 宋令荣[1] 赵永昌[1] 戴陆园[1] 鄢波[1] 黄兴奇[1] 

机构地区：[1]云南省农业科学院生物技术研究所,云南昆明650223

出　　处：《西南农业学报》2001年第4期17-19,共3页Southwest China Journal of Agricultural Sciences

基　　金：滇沪合作项目 ;云南省科学技术委员会资助

摘　　要：利用普通野生稻和疣粒野生稻去壳种子 ,诱导愈伤组织。愈伤组织用N6+ 1mg/L 2 ,4-D液体培养基进行悬浮培养继代。疣粒野生稻愈伤组织生长很快 ,每周至少继代培养 1次 ,有时甚至要继代 2次。疣粒野生稻愈伤组织继代培养 1年后分化能力仍然很强 ;普通野生稻愈伤组织继代 8个月后分化能力开始下降 ,至 10个月后分化能力完全丧失。在所用的 11种培养基中 ,N6+ 1mg/L 6 -BA + 0 5mg/LNAA诱导疣粒野生稻愈伤组织再生绿苗效果最好 ,诱导率为70 %。 1994年采集到的普通野生稻的种子 ,在本实验中 (1997年 )被用来诱导愈伤组织 ,其再生绿苗率平均仅为 4 %,比 1995年用该种子进行原生质体培养的再生率 (平均 2 3%)低 ,这可能与所用的诱导愈伤组织的种子保存时间 (3年 )太长有直接关系。Using the peeled and sterilized seeds as the materials of Oryza rufipogon Griff and Oryza meyeriana Baill their calli were induced on the solid medium(N 6+2 mg/L 2,4-D).For subculturing,the calli were suspended in the medium N 6 containing 1 mg/L 2,4-D,at least once a week.The calli from seeds O.meyeriana Baill grew faster than those from O.rufipogon Griff.After one years subculture,the former could regenerate green plants while the later not.Among 11 kinds of media used,the medium N 6 containing 1mg/L 6-BA+0.5 mg/L NAA,was optimum for regenerating green plants from the calli of O.meyeriana Baill.Its frequency of green plants was 70%.The frequencies of green plantlets from calli of O.rifipogon Griff was low (from 0% to 11%),and varied with different kinds of media used,which might be related to the long term of storage(three years)of seed.

关 键 词：组织培养 云南 野生稻 普通野生稻 疣粒野生稻 稻 

分 类 号：S511.903.5[农业科学—作物学]