黄芩甙 川芎嗪诱导大鼠脑组织热休克蛋白70合成  

Expression of Heat Shock Protein 70 Induced by Baicalin and Tetramethylphyrazine

作　　者：毛定安[1] 杨于嘉[2] 虞佩兰[2] 陶永光[2] 

机构地区：[1]中南大学湘雅二医院儿科 [2]中南大学湘雅医院儿科,湖南长沙410011

出　　处：《中国当代儿科杂志》2001年第4期367-369,共3页Chinese Journal of Contemporary Pediatrics

基　　金：国家自然科学基金项目 ( 39870 2 5 1)

摘　　要：目的　探讨黄芩甙、川芎嗪是否通过诱导热休克蛋白 (HSP70 )合成增加来保护脑组织。方法　SD大鼠随机分为①正常对照组 ,②热休克处理组 ,③感染性脑水肿组 ,④黄芩甙组 ,⑤川芎嗪组。其中黄芩甙和川芎嗪又分为小剂量、治疗量及大剂量各 3组。采用Western印迹杂交技术检测各组的HSP70的表达。结果　正常对照组、黄芩甙小剂量组、川芎嗪小剂量组及感染性脑水肿组均有一定量的HSP70合成 ,而黄芩甙、川芎嗪的治疗量及大剂量组以及热休克处理组均见明显的HSP70条带。结论　黄芩甙、川芎嗪能诱导脑组织HSP70合成明显增加 ,两药对感染性脑水肿的治疗机制可能与HSP70合成增加有关。Objective The previous experiments of our department had demonstrated that Baicalin (BC) and Tetramethylphyrazine (TMPZ) had protective effect against infectious brain edema in rats and rabbits. In the present study we would look for whether BC and TMPZ could influence HSP70 expression with heat shock responres(HSR). Methods Twenty-seven male rats were randomly divided into: ①NS group (n=3); ②HSR+pertusis bacillil (PB) group (n=3); ③PB group (n=3); ④BC group, in which the rats were subdivided into three groups (n=3 in each group) with BC injected intraperitoneal at 48 mg/kg, 120 mg/kg, 194 mg/kg respectively. ⑤TMPZ group, 9 rats were evaluated as follows, with 37.5 mg/kg, 100 mg/kg, 160 mg/kg TMPZ injected intraperitoneal respectively. The animals were killed after 28 hours and the expression of HSP 70 was evaluated by western blot analysis. Results HSP70 in the brain tissues was significantly elevated in the HS+PB group and 120 mg/kg BC, 194 mg/kg BC, 100 mg/kg TMPZ, 160 mg/kg TMPZ groups. HSP70 was only at base levels in the PB and NS groups and 48 mg/kg BC, 37.5 mg/kg TMPZ groups. Conclusions BC and TMPZ could influence HSP70 expression with HSR. The protective mechanism of BC and TMPZ on infectious brain edema in rats may be associated with the increase of HSP70 synthesis.

关 键 词：黄芩甙 川芎嗪 脑水肿 热休克蛋白 

分 类 号：R285.5[医药卫生—中药学]