印楝素对中华稻蝗若虫呼吸作用的影响  

The Effect of Azadirachtin on Respiration of Nymphs of Chinese Rice Grasshopper

作　　者：崔志新[1] 林进添[2] 赵善欢[2] 

机构地区：[1]佛山大学农牧学院,佛山528000 [2]华南农业大学农业部昆虫生态毒理重点开放实验室,广州510642

出　　处：《华中农业大学学报》2001年第6期544-546,共3页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目

摘　　要：呼吸测定试验表明 ,以每头印楝素 5 .0 μg点滴 3龄中华稻蝗蝗蝻前胸背板 ,4hCO2 释放量减少 ,抑制率达到 33%。对 6龄蝗蝻测定 ,处理后试虫CO2 释出量降低 ,呼吸受到抑制 ,而且随着时间的推移 ,呼吸失去了节律 ,图线变得近于直线。点滴法与注射法 ,在前期呼吸影响有所不同 ,点滴法随着经过的时间 ,呼吸抑制率提高 ;而注射法 4 8h呼吸抑制率才提高 ,到 72h达到最高 。From experiments of respiration of nymphs of Chinese rice grasshoppers (CRG), Oxya chinesensis (Thunberg) using of infrared CO 2 analyser, markedly decrease of CO 2 of CRG treated by Azadirachtin was observed. The respiratory rhythm was interfered greatly, no respiratory rhythm of the paralysed nymphs could be observed. The results indicated that the respiration of the nymphs treated by Azadirachtin was seriously hampered. The effects of Azadirachtin on the ecdysis and ecdysteroid titre of CRG were studied, and the results of RIA analysis showed that the haemolymph ecdysteroid titre of the adult was markedly decreased. This indicates that the ecdysteroid syntheses was inhibited. The inhibition is thought to be caused by the suppression of Azadirachtin on the neuroendocrine system of CRG.

关 键 词：印楝素 中华稻蝗 若虫 呼吸作用 致毒机理 

分 类 号：S435.112.1[农业科学—农业昆虫与害虫防治] S482.39[农业科学—植物保护]