低温下光照对黄瓜叶片光合特性的影响  

The Effect of Weak Light on Photosynthetic Properties of Cucumber Leaves at Low Temperature

作　　者：陈启林[1] 山仑[2] 程智慧[1] 

机构地区：[1]西北农林科技大学园艺学院,杨凌712100 [2]中国科学院水土保持研究所,杨凌712100

出　　处：《中国农业科学》2001年第6期632-636,共5页Scientia Agricultura Sinica

基　　金：国家重点基础研究发展规划项目(G1999011708)资助

摘　　要：为揭示低温下光照对黄瓜光合作用的影响，在叶片水平对５℃ １００μｍｏｌｍ·－’·ｓ－１和５℃黑暗处理４ｈ后黄瓜叶片光合－光响应和光合－ＣＯ２响应进行了对比研究、结果表明，５℃黑暗处理４ｈ使黄瓜叶片光饱和光合速率、表观光量子效率、ＣＯ２饱和光合速率以及梭化效率分别降低 ２２．５％、２８．０％、３１．３％和 ３７．２％，５℃ １００μｍｏｌ·ｍ－２·ｓ－１使上述指标分别降低 ５６．１％、６１．０％、５４．０％和 ５４．３％，说明 １００μｍｏｌ·ｍ－２·ｓ－１光照明显加剧５℃低温对黄瓜叶片光合功能的伤害。进一步研究结果显示低温光照处理使黄瓜叶片 ＰＳⅡ光合中心受到较严重的光抑制，类囊体跨膜质子梯度显著降低。对低温下光照对黄瓜叶片光合作用具有不良作用的原因进行了讨论。To investigate the effect of weak light on photosynthetic properties of cucumber leaves at low temperature, the effect of treatment with 5℃ darkness and 5℃ 100μmol·m-2·s-1 for 4h respectively on photosynthetic response to photon flux density(PFD) and to CO2 concentration were comparatively studied at leaf level. Light saturated photosynthetic rate, apparent quantum efficiency, CO2 saturated photosyn- thetic rate and carboxylation rate of cucumber leaves decreased by 22. 5 %, 28. 0%, 31' 3 % and 37. 2 % re- spectively after treatment at 5℃ darkness for 4h, and by 56. 1 %, 61. 0%, 54. 0% and 54. 3 % respectively after treatment with 5℃ 100μmol·m-2·s-1. The results indicated that exposure to 100μmol·m-2·s-1 at 5℃ caused worse damage to cucumber leaf photosynthetic function. Further results revealed that expo- sure of cucumber leaves to 100pmol·m-2·s-1 at 5℃ caused more serious PSⅡ photoinhibition and re- duced trans-thylakoidal proton gradient. The mechanism of damaging effect of weak light at low tempera- ture to cucumber leaf photosynthesis was discussed.

关 键 词：黄瓜 低温 光照 光合作用 叶片 

分 类 号：S642.2[农业科学—蔬菜学]