人胚肺细胞制备甲型肝炎抗原的研究  

STUDY ON PRODUCING HAAg WITH HUMAN FETAL LUNG CELLS

作　　者：尚守礼[1] 丛树梅 陈虹 张玉梅 徐伟 

机构地区：[1]山东医科大学传染病学教研室

出　　处：《山东医科大学学报》1991年第1期62-65,共4页Acta Academiae Medicinae Shandong

摘　　要：为生产甲型肝炎抗原,作者提出了一种新方法。采用人胚肺细胞代替2BS,接种HAV后进行离心培养和旋转培养。结果表明本法生产HAAg优于常规法:1.此种细胞价廉易得,对HAV敏感;2.HAV的增殖周期由2～3周缩短至7～10d;3.HAAg的产量增加近1倍。This paper presents a new technique for producing HAAg as the reagent of ELISA.HAV was inoculated in human fetal lung cells instead of 2 BS.Cultivation after centrifuging or rotating cultivation was succes- sively followed.In comparison with conventional method,this technique showed many advantages as:1.the cell line used in this study was cheaper, more susceptible to HAV,and easily available everywhere;2.the period of HAV propagation was shortened from 2—3 weeks to 7—10days;3.the vol- ume of HAAg production was nearly doubled。

关 键 词：人胚肺细胞 甲型肝炎病毒 甲型肝炎抗原 

分 类 号：R392－33[医药卫生—免疫学]