二色桌片参化学成分V. 二色桌片参糖蛋白抗肿瘤活性的研究  

Study on the Anti-tumor Activity of Glycoproteins GPMI-Ⅰand GPMI-ⅡExtracted from Sea Cucumber Mensamaria Intercedens

作　　者：陈粤[1] 苏文金[2] 吴萍茹 方金瑞 

机构地区：[1]汕头大学理学院生物学系,广东汕头515063 [2]厦门大学生命科学学院细胞生物学与肿瘤细胞工程教育部重点实验室 [3]福建海洋研究所

出　　处：《汕头大学医学院学报》2001年第4期251-252,265,共3页Journal of Shantou University Medical College

摘　　要：目的 :探讨二色桌片参糖蛋白GPMI Ⅰ和GPMI Ⅱ对小鼠移植性肿瘤S1 80 的抑制作用以及对荷瘤小鼠肝脾等器官重量的影响。方法 :通过对二色桌片参体壁进行水煮提取、分离、过SephadexG 2 0 0柱层析 ,获得二色桌片参糖蛋白Ⅰ (GPMI Ⅰ ) ,GPMI Ⅰ里的蛋白部分经酶解后得二色桌片参糖蛋白Ⅱ (GPMI Ⅱ )。结果 :GPMI Ⅰ在 30mg kg及 12 0mg kg剂量下可显著降低荷瘤小鼠的肝指数 (P <0 0 1,P <0 0 2 5 )。同时随着其剂量增加 ,抑瘤作用也明显增强 (P <0 0 0 1)。而经蛋白酶水解后的糖蛋白GPMI Ⅱ在剂量 2 0mg kg时对小鼠肉瘤S1 80 有较显著的抑制作用 (P <0 0 5 ) ,抑瘤率达 4 8 8%。同时发现 ,GP MI Ⅱ在 4 0mg kg剂量下能够显著地降低荷瘤小鼠肝指数 (P <0 0 5 )。结论 :GPMI Ⅰ和GPMI Ⅱ对小鼠S1 80Objective:To study the effects of two kinds of glycoproteins GPMI Ⅰand GPMI Ⅱ at inhibiting the growth of mice implanted tumor and the effects on the weight of spleen and liver of mice.Methods:GPMI Ⅰ and GPMI Ⅱ were extracted from sea cucumber mensamaria intercedens by boiling,separating and chromatography.Their anti tumor activity to mice implanted tumor Sarcoma 180 was evaluated in vitro by anti tumor assay.Results:GPMI Ⅱcould significantly inhibit the growth of Sarcoma 180 cells implanted subcutaneously in mice( P <0 05)at the dose of 20mg/kg per day×10 with no sign of toxicity.The ratio of tumor inhibition was 48 8% in Sarcoma 180 mice.GPMI Ⅱ decreased the weight of liver of mice with Sarcoma 180 cells at the dose of 40mg/kg per day×10.On the other hand,GPMI Ⅰdecreased the weight of liver of mice with Sarcoma 180 cells at the dose of 30mg/kg and 120mg/kg per day×10.GPMI Ⅰ could significantly inhibit the growth of Sarcoma 180 cells implanted subcutaneously in mice( P <0 001)at the dose of 120mg/kg per day×10 with no sign of toxicity.The effects appeared to be dose related.Conclusion:GPMI Ⅰ and GPMI Ⅱ can have significant effects on inhibition in Sarcoma 180 mice.

关 键 词：二色桌片参 糖蛋白 肉瘤S180 抑瘤作用 化学成分 低值海参 

分 类 号：R284.1[医药卫生—中药学] R285.5[医药卫生—中医学]