蝎毒活性多肽对内皮细胞释放PGI_2和NO的影响  

Effects of scorpion venom active peptides on the concentration of PGI_2 and NO secreated by human umbilical vein endothelical cells

作　　者：宋益民[1] 许兰芝[1] 李学坤[1] 郭方明[1] 高尔[1] 吕欣然[1] 

机构地区：[1]潍坊医学院药理学教研室,潍坊261042

出　　处：《中国临床药理学与治疗学》2001年第4期350-352,共3页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的　探讨蝎毒活性多肽 (SVAP)抗栓作用机制。方法　酶消化法培养人脐静脉内皮细胞 (HU VEC) ,放射免疫分析法测定HUVEC培养液中 6 Keto PGF1α的含量以反映PGI2 的变化 ,硝酸还原酶法测定NO的含量。结果　SVAP 1、5、10、2 0mg·L- 1均可明显增加HUVECPGI2 的释放 ,而SVAP 1、5mg·L- 1对NO的释放无明显影响 ,SVAP 10、2 0mg·L- 1可使NO释放量显著增加 ,PGI2 和NO释放量之间呈正相关。结论　SVAP抗栓机制可能与影响VEC合成或 (和 )分泌PGI2 ,NO以及NO与PGI2 二者之间协同及相互介导作用有关。Aim To further research into the antithrombotic mechanism of scorpion venom active peptides (SVAP). Methods Human umbilical vein endothelial cells (HUVEC) were cultured with enzyme digestive method. After the cultured HUVC was incubated in conditioned media for 1 hour, the effects of SVAP on the concentration of 6-Keto-PGF 1α and NO of HUVEC were determined with radioactive-immunolygic and nitrate reduction enzyme method respectively. Results As compared with control, SVAP in the doses of 1,5,10, 20 mg·L -1 had the distinctive increase of 54%, 68%,72%,79% of the concentration of 6-Keto-PGF 1α and SVAP in the doses of 10, 20 mg·L -1 had the significantly increased of 27%, 46% of the concentration of NO. Regression anylysis showed that the release levels of PGI 2 and NO in HUVEC induced by SVAP was of positive correlation. Conclusion Antithrombotic mechanism of SVAP is related to the increase of PGI 2 and NO released from HUVEC and synergistic and mediating action between NO and PGI 2.

关 键 词：蝎毒活性多肽 前列环素 一氧化氮 内皮细胞 

分 类 号：R331.3[医药卫生—人体生理学]