头孢唑啉降解产物在银亚微盘电极上的电化学行为及其测定  

Electrochemical Behavior of Degradation Product of Cefazolin with Silver Microdisk Electrode and Its Application to Analytical Chemistry

作　　者：张玉忠[1] 赵红[1] 袁倬斌[1] 

机构地区：[1]中国科学技术大学研究生院化学部,北京100039

出　　处：《分析化学》2002年第1期9-12,共4页Chinese Journal of Analytical Chemistry

摘　　要：基于头孢唑啉在 ０．１０ｍｏｌ／Ｌ ＮａＯＨ溶液中水浴降解产物中含有巯基（－ＳＨ），在 ｐＨ ５．０ＮａＡｃ－ＨＡｃ介质中，于银亚微盘电极上产生一灵敏的吸附伏安氧化还原峰，其峰电位分别为 Ｅｐａ＝－０．５３０Ｖ和 ＥＰｃ＝－０．４００Ｖ。动力学研究表明：电极反应的电子转移数为０．９１，表观电子传递速率为ｋｓ＝０．１０ｓ－１，峰电流与头孢唑啉浓度在１．０×１０－６～５０×１０－１ｍｏｌ／Ｌ范围内呈良好的线性关系。方法可应用于人工合成样品头孢啉的检测。The electrochemical behavior of degradation product of cefazolin (CFL) at silver sub-microdisk electrode has been studied. The degradation progress was carried out in 0.10 mol/L NaOH at 100degreesC for 20 minutes. The method was based on the degradation product of CFL containing thiohydroxy group (-SH), Which can combine with Ag+ to form an insoluble thiolate. In a medium, of 0.1 mol/L HAc-NaAc (pH 5.0), A pair of redox peaks were observed with the peak potential E-p1 = -0.53 V(reduction) and E-p2 = -0.40 V(oxidation). The electron-transfer rate constant (k(s)) and electron-transfer number are 0.10 s(-1) and 0.91 respectively at the scan rate of 100 mV/s. The linear range between the peak currents and the concentration of cefazolin was 5.0 x 10(-4) similar to 1.0 x 10(-6) mol/L. The electrode reaction process was quasi-reversible with adsorptive characteristic. This method has been successfully applied to the determination of CFL in simulate sample.

关 键 词：头孢唑啉 降解产物 银亚微盘电极 电化学行为 示差脉冲阴极溶出伏安法 测定 抗菌素 药物分析 

分 类 号：O646.56[理学—物理化学]